Literature summary for 2.3.1.117 extracted from

Simms, S.A.; Voige, W.H.; Gilvarg, C.
Purification and characterization of succinyl-CoA: tetrahydrodipicolinate N-succinyltransferase from Escherichia coli (1984), J. Biol. Chem., 259, 2734-2741.

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General Stability

General Stability	Organism
purification has to be carried out at 0-4°C	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
CaCl2	1 mM, 3% inhibition	Escherichia coli
CoCl2	1 mM, 95% inhibition	Escherichia coli
CuSO4	1 mM, complete loss of activity	Escherichia coli
MgCl2	1 mM, 20% inhibition	Escherichia coli
MnCl2	1 mM, 9% inhibition	Escherichia coli
additional information	not inhibited by EDTA and Fe2+	Escherichia coli
N-ethylmaleimide	16 mM, 42% inhibition	Escherichia coli
p-chloromercuriphenylsulfonate	0.16 mM, 97% inhibition	Escherichia coli
ZnCl2	1 mM, 88% inhibition	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	succinyl-CoA	-	Escherichia coli
0.022	-	tetrahydrodipicolinate	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	2 * 31000, SDS-PAGE	Escherichia coli
68000	-	gel filtration	Escherichia coli
72000	-	sucrose density gradient centrifugation	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
succinyl-CoA + (2S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylic acid	Escherichia coli	involved in the biosynthesis of lysine in bacteria, blue-green algae and higher plants	CoA + (2S)-2-[(3-carboxypropanoyl)amino]-6-oxoheptanedioic acid	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
MnCl2, ammonium sulfate, DEAE-cellulose, acid treatment, hydroxyapatite, phenyl-Sepharose, chromatofocusing	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
36	-	-	Escherichia coli

Storage Stability

Storage Stability	Organism
4°C, 0.001 M 2-mercaptoethanol, 80% remaining activity after 1 month	Escherichia coli
4°C, no 2-mercaptoethanol, 4 days, 80% loss of activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-CoA + (2S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylic acid	product formation is strongly preferred	Escherichia coli	CoA + (2S)-2-[(3-carboxypropanoyl)amino]-6-oxoheptanedioic acid	-	r
succinyl-CoA + (2S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylic acid	involved in the biosynthesis of lysine in bacteria, blue-green algae and higher plants	Escherichia coli	CoA + (2S)-2-[(3-carboxypropanoyl)amino]-6-oxoheptanedioic acid	-	r
succinyl-CoA + tetrahydrodipicolinate	-	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 31000, SDS-PAGE	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	-	-	Escherichia coli

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	50% of maximal activity at pH 7, 75% of maximal activity at pH 9.0	Escherichia coli

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Release 2023.1 (January 2023)