BRENDA - Enzyme Database show
show all sequences of 2.3.1.116

Two flavonoid-specific malonyltransferases from cell suspension cultures of Petroselinum hortense: partial purification and some properties of malonyl-coenzyme A: flavone/flavonol-7-O-glycoside malonyltransferase and malonyl-coenzyme A: flavonol-3-O-glucoside malonyltransferase

Matern, U.; Potts, J.R.M.; Hahlbrock, K.; Arch. Biochem. Biophys. 208, 233-241 (1981)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
kaempferol 3-O-glucoside
-
Petroselinum crispum
0.005
-
malonyl-CoA
-
Petroselinum crispum
0.006
-
isorhamnetin 3-O-glucoside
-
Petroselinum crispum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Petroselinum crispum
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtration
Petroselinum crispum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + kaempferol 3-O-glucoside
Petroselinum crispum
-
CoA + kaempferol 3-O-glucoside malonylester
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Petroselinum crispum
-
parsley
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Petroselinum crispum
Purification (Commentary)
Commentary
Organism
-
Petroselinum crispum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Petroselinum crispum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Petroselinum crispum
Storage Stability
Storage Stability
Organism
-70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days
Petroselinum crispum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + isorhamnetin 3-O-glucoside
78% of the activity with kaempferol 3-O-glucoside
486144
Petroselinum crispum
CoA + isorhamnetin 3-O-glucoside malonylester
probably acylated at position 6 of glucose
486144
Petroselinum crispum
?
malonyl-CoA + kaempferol 3-O-glucoside
-
486144
Petroselinum crispum
CoA + kaempferol 3-O-glucoside malonylester
-
-
-
?
malonyl-CoA + kaempferol 3-O-glucoside
-
486144
Petroselinum crispum
CoA + kaempferol 3-O-glucoside malonylester
probably acylated at position 6 of glucose
486144
Petroselinum crispum
?
malonyl-CoA + quercetin 3-O-glucoside
60% of the activity with kaempferol 3-O-glucoside
486144
Petroselinum crispum
CoA + quercetin 3-O-glucoside malonylester
probably acylated at position 6 of glucose
486144
Petroselinum crispum
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
most active in Tris buffer containing bovine serum albumin
Petroselinum crispum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
kaempferol 3-O-glucoside
-
Petroselinum crispum
0.005
-
malonyl-CoA
-
Petroselinum crispum
0.006
-
isorhamnetin 3-O-glucoside
-
Petroselinum crispum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Petroselinum crispum
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtration
Petroselinum crispum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + kaempferol 3-O-glucoside
Petroselinum crispum
-
CoA + kaempferol 3-O-glucoside malonylester
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Petroselinum crispum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Petroselinum crispum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Petroselinum crispum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Petroselinum crispum
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days
Petroselinum crispum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + isorhamnetin 3-O-glucoside
78% of the activity with kaempferol 3-O-glucoside
486144
Petroselinum crispum
CoA + isorhamnetin 3-O-glucoside malonylester
probably acylated at position 6 of glucose
486144
Petroselinum crispum
?
malonyl-CoA + kaempferol 3-O-glucoside
-
486144
Petroselinum crispum
CoA + kaempferol 3-O-glucoside malonylester
-
-
-
?
malonyl-CoA + kaempferol 3-O-glucoside
-
486144
Petroselinum crispum
CoA + kaempferol 3-O-glucoside malonylester
probably acylated at position 6 of glucose
486144
Petroselinum crispum
?
malonyl-CoA + quercetin 3-O-glucoside
60% of the activity with kaempferol 3-O-glucoside
486144
Petroselinum crispum
CoA + quercetin 3-O-glucoside malonylester
probably acylated at position 6 of glucose
486144
Petroselinum crispum
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
most active in Tris buffer containing bovine serum albumin
Petroselinum crispum
Other publictions for EC 2.3.1.116
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
662666
Suzuki
-
cDNA cloning and functional ch ...
Glandularia x hybrida, Lamium purpureum
J. Mol. Catal. B
28
87-93
2004
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
11
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
11
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
486143
Matern
Further characterization and r ...
Petroselinum crispum
Arch. Biochem. Biophys.
226
206-217
1983
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
486144
Matern
Two flavonoid-specific malonyl ...
Petroselinum crispum
Arch. Biochem. Biophys.
208
233-241
1981
-
-
-
-
-
-
-
3
1
-
1
1
-
1
-
1
1
-
-
1
1
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
1
1
-
-
1
1
-
1
1
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-