BRENDA - Enzyme Database show
show all sequences of 2.3.1.115

Nucleocytoplasmic-localized acyltransferases catalyze the malonylation of 7-O-glycosidic (iso)flavones in Medicago truncatula

Yu, X.; Chen, M.; Liu, C.; Plant J. 55, 382-396 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
health-promoting neutraceutical; health-promoting neutraceutical; health-promoting neutraceutical
Medicago truncatula
Cloned(Commentary)
Commentary
Organism
overexpression in Arabidopsis thaliana; overexpression in Arabidopsis thaliana; overexpression in Arabidopsis thaliana
Medicago truncatula
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0063
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
0.0078
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
0.0111
-
malonyl-CoA
-
Medicago truncatula
0.028
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.0422
-
malonyl-CoA
-
Medicago truncatula
0.0512
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.0713
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
0.1004
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.114
-
genistin
-
Medicago truncatula
0.1148
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.1256
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.1314
-
genistin
-
Medicago truncatula
0.3094
-
genistin
-
Medicago truncatula
0.5539
-
malonyl-CoA
-
Medicago truncatula
0.736
-
formononetin 7-O-glucoside
-
Medicago truncatula
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Medicago truncatula
5737
-
nucleus
-
Medicago truncatula
5634
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago truncatula
B4Y0U0
-
-
Medicago truncatula
B4Y0U1
-
-
Medicago truncatula
B4Y0U2
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Medicago truncatula
-
root
-
Medicago truncatula
-
stem
-
Medicago truncatula
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0017
-
-
Medicago truncatula
0.0058
-
-
Medicago truncatula
0.05
-
-
Medicago truncatula
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 6,4'-dihydroxy-7-O-glucosyl isoflavone
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + cyanidin 3,5-O-di-beta-D-glucoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + daidzin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + formononetin 7-O-glucoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + genistin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + hyperoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + isoflavone
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + isoquercitrin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + keracyanin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + kuromanin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + naringenin 7-O-glucoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + naringenin 7-O-neohesperidoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + rutin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at; assay at; assay at
Medicago truncatula
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Medicago truncatula
8
-
;
Medicago truncatula
Application (protein specific)
Application
Commentary
Organism
nutrition
health-promoting neutraceutical
Medicago truncatula
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Arabidopsis thaliana
Medicago truncatula
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0063
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
0.0078
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
0.0111
-
malonyl-CoA
-
Medicago truncatula
0.028
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.0422
-
malonyl-CoA
-
Medicago truncatula
0.0512
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.0713
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
0.1004
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.114
-
genistin
-
Medicago truncatula
0.1148
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.1256
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.1314
-
genistin
-
Medicago truncatula
0.3094
-
genistin
-
Medicago truncatula
0.5539
-
malonyl-CoA
-
Medicago truncatula
0.736
-
formononetin 7-O-glucoside
-
Medicago truncatula
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Medicago truncatula
5737
-
nucleus
-
Medicago truncatula
5634
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Medicago truncatula
-
root
-
Medicago truncatula
-
stem
-
Medicago truncatula
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0017
-
-
Medicago truncatula
0.0058
-
-
Medicago truncatula
0.05
-
-
Medicago truncatula
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 6,4'-dihydroxy-7-O-glucosyl isoflavone
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + cyanidin 3,5-O-di-beta-D-glucoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + daidzin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + formononetin 7-O-glucoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + genistin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + hyperoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + isoflavone
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + isoquercitrin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + keracyanin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + kuromanin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + naringenin 7-O-glucoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + naringenin 7-O-neohesperidoside
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
malonyl-CoA + rutin
assay at pH 8.0, 30°C
706233
Medicago truncatula
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Medicago truncatula
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Medicago truncatula
8
-
-
Medicago truncatula
Expression
Organism
Commentary
Expression
Medicago truncatula
decreased expression by copper treatment; decreased expression by drought stress; decreased expression by drought stress
down
Medicago truncatula
high expression in root and leaf, induced expression by copper treatment, slightly induced expression after elicitation of a root cell suspension culture by yeast elicitor; high expression in root and leaf, induced expression by copper treatment, slightly induced expression after elicitation of a root cell suspension culture by yeast elicitor; induced expression by drought stress and fungal infection
up
Expression (protein specific)
Organism
Commentary
Expression
Medicago truncatula
decreased expression by drought stress
down
Medicago truncatula
decreased expression by copper treatment
down
Medicago truncatula
high expression in root and leaf, induced expression by copper treatment, slightly induced expression after elicitation of a root cell suspension culture by yeast elicitor
up
Medicago truncatula
induced expression by drought stress and fungal infection
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.08
-
genistin
-
Medicago truncatula
0.0906
-
genistin
-
Medicago truncatula
0.1152
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.2308
-
malonyl-CoA
-
Medicago truncatula
0.3365
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.6249
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.948
-
naringenin 7-O-glucoside
-
Medicago truncatula
1.01
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
1.52
-
formononetin 7-O-glucoside
-
Medicago truncatula
1.679
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
3.2
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
3.89
-
naringenin 7-O-glucoside
-
Medicago truncatula
3.9
-
malonyl CoA
-
Medicago truncatula
3.952
-
malonyl-CoA
-
Medicago truncatula
6.57
-
genistin
-
Medicago truncatula
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.08
-
genistin
-
Medicago truncatula
0.0906
-
genistin
-
Medicago truncatula
0.1152
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.2308
-
malonyl-CoA
-
Medicago truncatula
0.3365
-
formononetin 7-O-glucoside
-
Medicago truncatula
0.6249
-
naringenin 7-O-glucoside
-
Medicago truncatula
0.948
-
naringenin 7-O-glucoside
-
Medicago truncatula
1.01
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
1.52
-
formononetin 7-O-glucoside
-
Medicago truncatula
1.679
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
3.2
-
naringenin 7-O-neohesperidoside
-
Medicago truncatula
3.89
-
naringenin 7-O-glucoside
-
Medicago truncatula
3.9
-
malonyl CoA
-
Medicago truncatula
3.952
-
malonyl-CoA
-
Medicago truncatula
6.57
-
genistin
-
Medicago truncatula
Other publictions for EC 2.3.1.115
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
706233
Yu
Nucleocytoplasmic-localized ac ...
Medicago truncatula
Plant J.
55
382-396
2008
-
1
1
-
-
-
-
15
2
-
-
-
-
6
-
-
-
-
-
3
3
-
39
-
1
-
-
-
2
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
15
6
-
-
-
-
-
-
-
-
9
3
-
39
-
3
-
-
-
3
-
-
-
2
-
-
6
15
15
486145
Koester
Malonyl-coenzyme A:isoflavone ...
Cicer arietinum
Arch. Biochem. Biophys.
234
513-521
1984
-
-
-
-
-
1
-
15
-
-
1
1
-
1
-
-
1
-
-
1
1
1
16
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
15
-
-
1
1
-
-
-
1
-
1
1
1
16
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
486143
Matern
Further characterization and r ...
Petroselinum crispum
Arch. Biochem. Biophys.
226
206-217
1983
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
486144
Matern
Two flavonoid-specific malonyl ...
Petroselinum crispum
Arch. Biochem. Biophys.
208
233-241
1981
-
-
-
-
-
-
-
4
1
-
1
2
-
1
-
1
1
-
-
1
1
1
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
1
2
-
-
1
1
-
1
1
1
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-