BRENDA - Enzyme Database show
show all sequences of 2.3.1.115

Malonyl-coenzyme A:isoflavone 7-O-glucoside-6-O-malonyltransferase from roots of chick pea (Cicer arietinum L.)

Koester, J.; Bussmann, R.; Barz, W.; Arch. Biochem. Biophys. 234, 513-521 (1984)

Data extracted from this reference:

General Stability
General Stability
Organism
most stable in Tris buffer at pH 8, unstable to repeated freezing and thawing, dithioerythritol stabilizes
Cicer arietinum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
formononetin
-
Cicer arietinum
0.036
-
Biochanin A
-
Cicer arietinum
0.048
-
malonyl-CoA
-
Cicer arietinum
0.054
-
(-)-maackiain 3-O-beta-D-glucoside
-
Cicer arietinum
0.065
-
daidzein 7-O-glucoside
-
Cicer arietinum
0.067
-
kaempferol 7-O-glucoside
-
Cicer arietinum
0.088
-
genistein 7-O-glucoside
-
Cicer arietinum
0.098
-
pratensein 7-O-glucoside
-
Cicer arietinum
0.101
-
succinyl-CoA
-
Cicer arietinum
0.11
-
2',4,4'-trihydroxychalcone 4'-O-glucoside
-
Cicer arietinum
0.16
-
Apigenin 7-O-glucoside
-
Cicer arietinum
0.335
-
quercetagetin 7-O-glucoside
-
Cicer arietinum
0.381
-
quercetin 7-O-glucoside
-
Cicer arietinum
0.46
-
orobol 7-O-glucoside
-
Cicer arietinum
0.47
-
Luteolin 7-O-glucoside
-
Cicer arietinum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
112000
-
gel filtration
Cicer arietinum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
apigenin 7-O-apiosylglucoside + malonyl-CoA
Cicer arietinum
i.e. apiin
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
Cicer arietinum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cicer arietinum
-
chick pea
-
Purification (Commentary)
Commentary
Organism
-
Cicer arietinum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
4 days old
Cicer arietinum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.515
-
-
Cicer arietinum
Storage Stability
Storage Stability
Organism
-20°C, 10% glycerol, stable for 4 months
Cicer arietinum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
apigenin 7-O-apiosylglucoside + malonyl-CoA
i.e. apiin
486145
Cicer arietinum
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
486145
Cicer arietinum
?
apigenin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
-
-
?
luteolin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl CoA + genistein 7-O-glucoside
-
486145
Cicer arietinum
CoA + genistein 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + (-)-maackiain 3-O-beta-D-glucoside
-
486145
Cicer arietinum
CoA + (-)-maackiain 3-O-(6-O-malonyl)-beta-D-glucoside
-
-
-
?
malonyl-CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside
-
486145
Cicer arietinum
CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + biochanin A 7-O-glucoside
-
486145
Cicer arietinum
CoA + biochanin A 7-O-glucoside-6''-O-malonylester
-
486145
Cicer arietinum
?
malonyl-CoA + daidzein 7-O-glucoside
-
486145
Cicer arietinum
CoA + daidzein 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + formononetin 7-O-glucoside
-
486145
Cicer arietinum
CoA + formononetin 7-O-glucoside-6''-O-malonylester
-
486145
Cicer arietinum
?
malonyl-CoA + kaempferol 7-O-glucoside
-
486145
Cicer arietinum
CoA + kaempferol 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + orobol 7-O-glucoside
-
486145
Cicer arietinum
CoA + orobol 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + pratensein 7-O-glucoside
-
486145
Cicer arietinum
CoA + pratensein 7-O-glucoside 6''-O-malonylester
-
-
-
?
additional information
isoflavones with the glucosyl substituent in position 4' don't act as acceptors
486145
Cicer arietinum
?
-
-
-
-
additional information
succinyl-CoA can replace malonyl-CoA with lower activity
486145
Cicer arietinum
?
-
-
-
-
quercetagetin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + quercetagetin 7-O-glucoside 6''-O-malonylester
-
-
-
?
quercetin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + quercetin 7-O-glucoside 6''-O-malonylester
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
50
-
Cicer arietinum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
above 2 min, destroyed
Cicer arietinum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Cicer arietinum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
about 50% of activity maximum at pH 6.5 and 9.5
Cicer arietinum
General Stability (protein specific)
General Stability
Organism
most stable in Tris buffer at pH 8, unstable to repeated freezing and thawing, dithioerythritol stabilizes
Cicer arietinum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
formononetin
-
Cicer arietinum
0.036
-
Biochanin A
-
Cicer arietinum
0.048
-
malonyl-CoA
-
Cicer arietinum
0.054
-
(-)-maackiain 3-O-beta-D-glucoside
-
Cicer arietinum
0.065
-
daidzein 7-O-glucoside
-
Cicer arietinum
0.067
-
kaempferol 7-O-glucoside
-
Cicer arietinum
0.088
-
genistein 7-O-glucoside
-
Cicer arietinum
0.098
-
pratensein 7-O-glucoside
-
Cicer arietinum
0.101
-
succinyl-CoA
-
Cicer arietinum
0.11
-
2',4,4'-trihydroxychalcone 4'-O-glucoside
-
Cicer arietinum
0.16
-
Apigenin 7-O-glucoside
-
Cicer arietinum
0.335
-
quercetagetin 7-O-glucoside
-
Cicer arietinum
0.381
-
quercetin 7-O-glucoside
-
Cicer arietinum
0.46
-
orobol 7-O-glucoside
-
Cicer arietinum
0.47
-
Luteolin 7-O-glucoside
-
Cicer arietinum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
112000
-
gel filtration
Cicer arietinum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
apigenin 7-O-apiosylglucoside + malonyl-CoA
Cicer arietinum
i.e. apiin
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
Cicer arietinum
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cicer arietinum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
4 days old
Cicer arietinum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.515
-
-
Cicer arietinum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10% glycerol, stable for 4 months
Cicer arietinum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
apigenin 7-O-apiosylglucoside + malonyl-CoA
i.e. apiin
486145
Cicer arietinum
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
486145
Cicer arietinum
?
apigenin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
-
-
?
luteolin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl CoA + genistein 7-O-glucoside
-
486145
Cicer arietinum
CoA + genistein 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + (-)-maackiain 3-O-beta-D-glucoside
-
486145
Cicer arietinum
CoA + (-)-maackiain 3-O-(6-O-malonyl)-beta-D-glucoside
-
-
-
?
malonyl-CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside
-
486145
Cicer arietinum
CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + biochanin A 7-O-glucoside
-
486145
Cicer arietinum
CoA + biochanin A 7-O-glucoside-6''-O-malonylester
-
486145
Cicer arietinum
?
malonyl-CoA + daidzein 7-O-glucoside
-
486145
Cicer arietinum
CoA + daidzein 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + formononetin 7-O-glucoside
-
486145
Cicer arietinum
CoA + formononetin 7-O-glucoside-6''-O-malonylester
-
486145
Cicer arietinum
?
malonyl-CoA + kaempferol 7-O-glucoside
-
486145
Cicer arietinum
CoA + kaempferol 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + orobol 7-O-glucoside
-
486145
Cicer arietinum
CoA + orobol 7-O-glucoside 6''-O-malonylester
-
-
-
?
malonyl-CoA + pratensein 7-O-glucoside
-
486145
Cicer arietinum
CoA + pratensein 7-O-glucoside 6''-O-malonylester
-
-
-
?
additional information
isoflavones with the glucosyl substituent in position 4' don't act as acceptors
486145
Cicer arietinum
?
-
-
-
-
additional information
succinyl-CoA can replace malonyl-CoA with lower activity
486145
Cicer arietinum
?
-
-
-
-
quercetagetin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + quercetagetin 7-O-glucoside 6''-O-malonylester
-
-
-
?
quercetin 7-O-glucoside + malonyl-CoA
-
486145
Cicer arietinum
CoA + quercetin 7-O-glucoside 6''-O-malonylester
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
50
-
Cicer arietinum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
above 2 min, destroyed
Cicer arietinum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Cicer arietinum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
about 50% of activity maximum at pH 6.5 and 9.5
Cicer arietinum
Other publictions for EC 2.3.1.115
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
706233
Yu
Nucleocytoplasmic-localized ac ...
Medicago truncatula
Plant J.
55
382-396
2008
-
1
1
-
-
-
-
15
2
-
-
-
-
6
-
-
-
-
-
3
3
-
39
-
1
-
-
-
2
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
15
6
-
-
-
-
-
-
-
-
9
3
-
39
-
3
-
-
-
3
-
-
-
2
-
-
6
15
15
486145
Koester
Malonyl-coenzyme A:isoflavone ...
Cicer arietinum
Arch. Biochem. Biophys.
234
513-521
1984
-
-
-
-
-
1
-
15
-
-
1
1
-
1
-
-
1
-
-
1
1
1
16
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
15
-
-
1
1
-
-
-
1
-
1
1
1
16
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
486143
Matern
Further characterization and r ...
Petroselinum crispum
Arch. Biochem. Biophys.
226
206-217
1983
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
486144
Matern
Two flavonoid-specific malonyl ...
Petroselinum crispum
Arch. Biochem. Biophys.
208
233-241
1981
-
-
-
-
-
-
-
4
1
-
1
2
-
1
-
1
1
-
-
1
1
1
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
1
2
-
-
1
1
-
1
1
1
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-