BRENDA - Enzyme Database show
show all sequences of 2.3.1.112

N-Malonyltransferases from peanut

Matern, U.; Feser, C.; Heller, W.; Arch. Biochem. Biophys. 235, 218-227 (1984)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
bovine serum albumin
2 mg/ml, activation
Arachis hypogaea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
malonyl-CoA
-
Arachis hypogaea
0.025
-
D-tryptophan
-
Arachis hypogaea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
1 * 38000, SDS-PAGE
Arachis hypogaea
40000
-
gel filtration
Arachis hypogaea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + D-tryptophan
Arachis hypogaea
-
CoA + N2-malonyl-D-tryptophan
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arachis hypogaea
-
peanut, cv. fastigiata (Junagarh-11 or cultivar Shulamit)
-
Purification (Commentary)
Commentary
Organism
partial
Arachis hypogaea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Arachis hypogaea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0002
-
-
Arachis hypogaea
Storage Stability
Storage Stability
Organism
-20°C, inactivation after 3 months
Arachis hypogaea
4°C, considerable loss of activity after several weeks
Arachis hypogaea
glycerol, 10%, stabilizes during storage
Arachis hypogaea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 1-aminocyclopropan-1-carboxylic acid
condensation at 50% the rate of the condensation with D-tryptophan
486140
Arachis hypogaea
CoA + malonyl-1-aminocyclopropan-1-carboxylic acid
-
-
-
?
malonyl-CoA + D-tryptophan
-
486140
Arachis hypogaea
CoA + N2-malonyl-D-tryptophan
-
-
-
?
additional information
no substrates are L-tryptophan, anthranilate and 3,4-dichloroaniline
486140
Arachis hypogaea
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Arachis hypogaea
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arachis hypogaea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
broad
Arachis hypogaea
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
most active
Arachis hypogaea
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
bovine serum albumin
2 mg/ml, activation
Arachis hypogaea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
malonyl-CoA
-
Arachis hypogaea
0.025
-
D-tryptophan
-
Arachis hypogaea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
1 * 38000, SDS-PAGE
Arachis hypogaea
40000
-
gel filtration
Arachis hypogaea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + D-tryptophan
Arachis hypogaea
-
CoA + N2-malonyl-D-tryptophan
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Arachis hypogaea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Arachis hypogaea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0002
-
-
Arachis hypogaea
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, inactivation after 3 months
Arachis hypogaea
4°C, considerable loss of activity after several weeks
Arachis hypogaea
glycerol, 10%, stabilizes during storage
Arachis hypogaea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 1-aminocyclopropan-1-carboxylic acid
condensation at 50% the rate of the condensation with D-tryptophan
486140
Arachis hypogaea
CoA + malonyl-1-aminocyclopropan-1-carboxylic acid
-
-
-
?
malonyl-CoA + D-tryptophan
-
486140
Arachis hypogaea
CoA + N2-malonyl-D-tryptophan
-
-
-
?
additional information
no substrates are L-tryptophan, anthranilate and 3,4-dichloroaniline
486140
Arachis hypogaea
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Arachis hypogaea
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arachis hypogaea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
broad
Arachis hypogaea
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
most active
Arachis hypogaea
Other publictions for EC 2.3.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486140
Matern
N-Malonyltransferases from pea ...
Arachis hypogaea
Arch. Biochem. Biophys.
235
218-227
1984
1
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
1
1
3
3
1
1
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
3
3
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-