Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Capsicum annuum |
Protein Variants | Comment | Organism |
---|---|---|
F145Y | KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 3fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin | Capsicum annuum |
F145Y/Y147F | KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 4.2fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin | Capsicum annuum |
additional information | a series of chimeric genes is constructed by reciprocal exchange of DNA segments between the serotonin N-hydroxycinnamoyltransferase and tyramine N-hydroxycinnamoyltransferase cDNAs. Functional characterization of the recombinant chimeric proteins revealed that the amino acid residues 129 to 165 of serotonin N-hydroxycinnamoyltransferase and the corresponding residues 125 to 160 in tyramine N-hydroxycinnamoyltransferase are critical structural determinants for amine substrate specificity | Capsicum annuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-coumaroyl-CoA | 30°C, pH 8.5, recombinant enzyme | Capsicum annuum | |
0.04 | - |
tyramine | 30°C, pH 8.5, recombinant enzyme | Capsicum annuum | |
0.053 | - |
feruloyl-CoA | 30°C, pH 8.5, recombinant enzyme | Capsicum annuum | |
0.17 | - |
tyramine | 30°C, pH 8.5, mutant enzyme F145Y | Capsicum annuum | |
0.17 | - |
tyramine | 30°C, pH 8.5, mutant enzyme F145Y/Y147F | Capsicum annuum | |
0.779 | - |
dopamine | 30°C, pH 8.5, recombinant enzyme | Capsicum annuum | |
20.98 | - |
serotonin | 30°C, pH 8.5, mutant enzyme F145Y | Capsicum annuum | |
24 | - |
tryptamine | 30°C, pH 8.5, recombinant enzyme | Capsicum annuum | |
25.44 | - |
serotonin | 30°C, pH 8.5, mutant enzyme F145Y/Y147F | Capsicum annuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28221 | - |
x * 28221, calculatred from nucleotide sequence | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | Q5D8C0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | THT mRNA is expressed constitutively in all pepper organs | Capsicum annuum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + tyramine | - |
Capsicum annuum | CoA + N-(4-coumaroyl)tyramine | - |
? | |
caffeoyl-CoA + serotonin | - |
Capsicum annuum | CoA + N-caffeoylserotonin | - |
? | |
feruloyl-CoA + dopamine | - |
Capsicum annuum | CoA + feruloyldopamine | - |
? | |
feruloyl-CoA + tryptamine | - |
Capsicum annuum | CoA + feruloyltryptamine | - |
? | |
feruloyl-CoA + tyramine | - |
Capsicum annuum | CoA + feruloyltyramine | - |
? | |
additional information | wild-type enzyme shows no activity with serotonin, mutant enzyms F145Y and F145Y/Y147F show activity with serotonin | Capsicum annuum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28221, calculatred from nucleotide sequence | Capsicum annuum |
Synonyms | Comment | Organism |
---|---|---|
THT | - |
Capsicum annuum |
tyramine N-hydroxycinnamoyltransferase | - |
Capsicum annuum |