Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-arginine | allosteric activator of the ornithine succinyltransferase activity | Pseudomonas aeruginosa | |
L-lysine | activator of the ornithine succinyltransferase activity | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
agmatine | - |
Pseudomonas aeruginosa | |
D-arginine | competitive inhibition | Pseudomonas aeruginosa | |
spermidine | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-ornithine | - |
Pseudomonas aeruginosa | |
0.5 | - |
L-arginine | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE | Pseudomonas aeruginosa |
37000 | - |
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE | Pseudomonas aeruginosa |
140000 | - |
PAGE | Pseudomonas aeruginosa |
150000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + L-arginine | Pseudomonas aeruginosa | - |
CoA + N2-succinyl-L-arginine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, retains full activity with both substrates for several weeks, loses 10-40% of its activity following repeated freezing and thawing | Pseudomonas aeruginosa |
4°C, 10 mM potassium phosphate, pH 7.5, stable for 48 h | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | D-arginine, D-ornithine, L-lysine, L-citrulline, 2,4-aminobutyrate, 5-aminovalerate, 2-aminovalerate, agmatine or the polyamines propanediamine, putrescine, cadaverine and spermidine are not substrates | Pseudomonas aeruginosa | ? | - |
? | |
succinyl-CoA + L-arginine | - |
Pseudomonas aeruginosa | CoA + N2-succinyl-L-arginine | - |
? | |
succinyl-CoA + L-homoarginine | - |
Pseudomonas aeruginosa | CoA + N2-succinyl-L-homoarginine | - |
? | |
succinyl-CoA + L-ornithine | - |
Pseudomonas aeruginosa | CoA + N2-succinyl-L-ornithine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
L-homoarginine as substrate | Pseudomonas aeruginosa |
8.7 | - |
L-arginine as substrate | Pseudomonas aeruginosa |
9 | - |
L-ornithine as substrate | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
spermidine | - |
Pseudomonas aeruginosa | |
2.6 | - |
D-arginine | - |
Pseudomonas aeruginosa | |
3.4 | - |
agmatine | - |
Pseudomonas aeruginosa |