Cloned (Comment) | Organism |
---|---|
expression of GST-tagged Mec-17 in Escherichia coli strain BL21 | Mus musculus |
MEC-17 sequences are absent from Chlamydomonas reinhardtii, an organism that has alphaTAT activity | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
flagellum | - |
Chlamydomonas reinhardtii | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [alpha-tubulin]-L-lysine | Chlamydomonas reinhardtii | acetylation of the epsilon-amino group of Lys40 | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | Mus musculus | acetylation of the epsilon-amino group of Lys40 | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | Caenorhabditis elegans | acetylation of the epsilon-amino group of Lys40 | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | Danio rerio | acetylation of the epsilon-amino group of Lys40 | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | Tetrahymena thermophila | acetylation of the epsilon-amino group of Lys40, in Tetrahymena, alpha-tubulin is the major if not the only substrate of MEC-17-dependent K acetylation | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
additional information | Caenorhabditis elegans | wild-type adults have a strong signal for acetylated alpha-tubulin in the six touch receptor neurons | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Chlamydomonas reinhardtii | - |
- |
- |
Danio rerio | - |
- |
- |
Mus musculus | - |
- |
- |
Tetrahymena thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged Mec-17 from Escherichia coli strain BL21 by glutathione affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Danio rerio | - |
neuron | peripheral, acetyl-K40 alpha-tubulin is enriched in cilia and axons of neurons | Danio rerio | - |
optic nerve | - |
Danio rerio | - |
spinal cord | - |
Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of the epsilon-amino group of Lys40 | Chlamydomonas reinhardtii | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of the epsilon-amino group of Lys40 | Mus musculus | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of the epsilon-amino group of Lys40 | Caenorhabditis elegans | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of the epsilon-amino group of Lys40 | Tetrahymena thermophila | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of the epsilon-amino group of Lys40 | Danio rerio | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of the epsilon-amino group of Lys40, in Tetrahymena, alpha-tubulin is the major if not the only substrate of MEC-17-dependent K acetylation | Tetrahymena thermophila | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
additional information | wild-type adults have a strong signal for acetylated alpha-tubulin in the six touch receptor neurons | Caenorhabditis elegans | ? | - |
? | |
additional information | in vitro, MEC-17 exclusively acetylates Lys40 of alpha-tubulin | Caenorhabditis elegans | ? | - |
? | |
additional information | recombinant GST-MEC-17 directly acetylates purified tubulin from the MEC17-KO strain in vitro | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-TAT | - |
Chlamydomonas reinhardtii |
alpha-TAT | - |
Mus musculus |
alpha-TAT | - |
Caenorhabditis elegans |
alpha-TAT | - |
Tetrahymena thermophila |
alpha-TAT | - |
Danio rerio |
alpha-tubulin acetyltransferase | - |
Chlamydomonas reinhardtii |
alpha-tubulin acetyltransferase | - |
Mus musculus |
alpha-tubulin acetyltransferase | - |
Caenorhabditis elegans |
alpha-tubulin acetyltransferase | - |
Tetrahymena thermophila |
alpha-tubulin acetyltransferase | - |
Danio rerio |
Mec-17 | - |
Mus musculus |
Mec-17 | - |
Caenorhabditis elegans |
Mec-17 | - |
Tetrahymena thermophila |
Mec-17 | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Chlamydomonas reinhardtii |
8 | - |
assay at | Mus musculus |
8 | - |
assay at | Caenorhabditis elegans |
8 | - |
assay at | Tetrahymena thermophila |
8 | - |
assay at | Danio rerio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Chlamydomonas reinhardtii | |
acetyl-CoA | - |
Mus musculus | |
acetyl-CoA | - |
Caenorhabditis elegans | |
acetyl-CoA | - |
Tetrahymena thermophila | |
acetyl-CoA | - |
Danio rerio |
Organism | Comment | Expression |
---|---|---|
Tetrahymena thermophila | construction of a Mec-17 disruption mutant. The MEC-17-KO Tetrahymena cells have a normal growth rate, but theMEC-17-KOcells grow more slowly than wild type on medium with the microtubule depolymerizing compound oryzalin. Conversely, the MEC-17 KO cells grew faster than wild-type cells in medium with paclitaxel, a microtubule-stabilizing drug. This drug phenotype is consistent with an increase in dynamics of microtubules in MEC17-KO cells | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | MEC-17 is related to the Gcn5 histone acetyltransferases | Mus musculus |
evolution | MEC-17 is related to the Gcn5 histone acetyltransferases | Caenorhabditis elegans |
evolution | MEC-17 is related to the Gcn5 histone acetyltransferases | Tetrahymena thermophila |
evolution | MEC-17 is related to the Gcn5 histone acetyltransferases | Danio rerio |
malfunction | disruption of the Tetrahymena MEC-17 gene, resulting in a marked loss of acetyl-K40 in Tetrahymena cells, phenocopies the K40R alpha-tubulin mutation and makes microtubules more labile. Overexpression of GFP-Mec17p in Tetrahymena greatly increases acetylation of microtubules | Tetrahymena thermophila |
malfunction | MEC-12 is the only alpha-tubulin with K40, and mec-12(e1607) probable null allele worms have greatly reduced touch responses. Intergration of single transgenes encoding MEC-12 with either wild-type K40 or K40R or K40Q substitutions into the mec-12(e1607)mutant using Mos1 transposon excision repair restores the levels of touch response to 80% of wild type level,whereas animals with either MEC-12-K40R or MEC-12-K40Q show reduced touch response, overview | Caenorhabditis elegans |
malfunction | zebrafish embryos depleted in MEC-17 show a dramatic loss of acetyl-K40 in neurons but not in cilia. Depletion of MEC-17 in zebrafish, by injection with random sequence morpholinos or 5-bp mismatched morpholinos, produces phenotypes consistent with neuromuscular defects | Danio rerio |
additional information | MEC-17 sequences are absent from Chlamydomonas reinhardtii, an organism that has alphaTAT activity | Chlamydomonas reinhardtii |
physiological function | MEC-17 is required for the function of touch receptor neurons in Caenorhabditis elegans and acts as a K40-specific acetyltransferase for alpha-tubulin. W06B11.1 os also required for acetylation of K40 and contribute to touch sensation | Caenorhabditis elegans |
physiological function | the K40 residue of alpha-tubulin is important in vertebrates, acetyl-K40-carrying microtubules are abundant in the nervous system, including the brain, optical nerves, spinal cord, and axons of peripheral nerves. MEC-17 is required for K40 acetylation in zebrafish and normal embryonic development | Danio rerio |
physiological function | the K40 residue of alpha-tubulin is important in vertebrates, MEC-17 controls the levels of microtubule acetylation in mammalian cells | Mus musculus |
physiological function | the K40 residue of alpha-tubulin is not required for survival in protists, such as Chlamydomonas | Chlamydomonas reinhardtii |
physiological function | the K40 residue of alpha-tubulin is not required for survival in protists, such as Tetrahymena | Tetrahymena thermophila |