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Literature summary for 2.3.1.107 extracted from
- Expression of deacetylvindoline-4-O-acetyltransferase in Catharanthus roseus hairy roots (2007), Phytochemistry, 68, 1922-1931.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli and Catharanthus roseus | Catharanthus roseus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | DAT is overexpressed in Catharanthus roseus hairy root cultures: metabolite analysis using HPLC show that transgenic hairy root extracts have an altered alkaloid profile with respect to horhammericine accumulation. It is concluded that expression of DAT in hairy roots alters their monoterpenoid indole alkaloids profile. Evidence is provided that horhammericine accumulates via a DAT interaction with the root specific minovincinine-19-O-acetyltransferase (MAT) that inhibits the MAT mediated conversion of horhammericine into 19-O-acetyl-horhammericine | Catharanthus roseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| minovincinine-19-O-acetyltransferase | incubation of DAT with varying amounts of crude recombinant minovincinine-19-O-acetyltransferase (MAT) inhibits DAT activity in a dose dependent manner | Catharanthus roseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hair root | - |
Catharanthus roseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + deacetylvindoline | - |
Catharanthus roseus | CoA + vindoline | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAT | - |
Catharanthus roseus |
| deacetylvindoline-4-O-acetyltransferase | - |
Catharanthus roseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Catharanthus roseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Catharanthus roseus | |
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Release 2023.1 (January 2023)
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