Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 47.8% activity in absence of both EDTA and mercaptoethanol | Oryctolagus cuniculus | |
mercaptoethanol | 47.8% activity in absence of both EDTA and mercaptoethanol | Oryctolagus cuniculus | |
additional information | activity increases in 2.4fold by preincubation with ATP, MgCl2 and high speed supernatant fractions | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 29% decline in subcellular fraction P3A activity, 43% decline in subcellular fraction P3B activity and 7% decline in subcellular fraction P3D activity | Oryctolagus cuniculus | |
Alkaline phosphatase | 92-95% activity lost for subcellular fractions P3A and P3B | Oryctolagus cuniculus | |
ATP | little effect on the enzyme activity for the subcellular fraction P3A, 55-64% decline in the subcellular fraction P3B | Oryctolagus cuniculus | |
CaCl2 | 1 mM CaCl2 inhibits 73% and 24% activity of subcellular fractions P3B and P3D respectively | Oryctolagus cuniculus | |
EDTA | inhibits 30% fraction P3D, does not inhibit subcellular fraction P3A | Oryctolagus cuniculus | |
MgADP- | inhibitory effects less severe than MgATP | Oryctolagus cuniculus | |
MgAMP | less inhibitory effect than MgADP | Oryctolagus cuniculus | |
MgATP2- | extreme inhibition in all microsomal fractions | Oryctolagus cuniculus | |
MgCl2 | at 2 mM: 30, 28 and 58% inhibition of subcellular fractions P3A, P3B and P3D activities respectively | Oryctolagus cuniculus | |
additional information | ATP, GTP, UTP, CTP show small or no inhibition effects for fraction P3A | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate | Oryctolagus cuniculus | - |
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isolation of three subcellular fractions: P3A, P3B and P3D, using NaF, EDTA and mercaptoethanol to preserve the activity | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cerebral cortex | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate | - |
Oryctolagus cuniculus | CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate | - |
? |