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show all sequences of 2.3.1.105

MgATP inhibits the synthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphate by microsomal acetyltransferase of immature rabbit cerebral cortex

Baker, R.R.; Chang, H.Y.; Biochim. Biophys. Acta 1213, 27-33 (1994)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
47.8% activity in absence of both EDTA and mercaptoethanol
Oryctolagus cuniculus
mercaptoethanol
47.8% activity in absence of both EDTA and mercaptoethanol
Oryctolagus cuniculus
additional information
activity increases in 2.4fold by preincubation with ATP, MgCl2 and high speed supernatant fractions
Oryctolagus cuniculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
29% decline in subcellular fraction P3A activity, 43% decline in subcellular fraction P3B activity and 7% decline in subcellular fraction P3D activity
Oryctolagus cuniculus
Alkaline phosphatase
92-95% activity lost for subcellular fractions P3A and P3B
Oryctolagus cuniculus
ATP
little effect on the enzyme activity for the subcellular fraction P3A, 55-64% decline in the subcellular fraction P3B
Oryctolagus cuniculus
CaCl2
1 mM CaCl2 inhibits 73% and 24% activity of subcellular fractions P3B and P3D respectively
Oryctolagus cuniculus
EDTA
inhibits 30% fraction P3D, does not inhibit subcellular fraction P3A
Oryctolagus cuniculus
MgADP-
inhibitory effects less severe than MgATP
Oryctolagus cuniculus
MgAMP
less inhibitory effect than MgADP
Oryctolagus cuniculus
MgATP2-
extreme inhibition in all microsomal fractions
Oryctolagus cuniculus
MgCl2
at 2 mM: 30, 28 and 58% inhibition of subcellular fractions P3A, P3B and P3D activities respectively
Oryctolagus cuniculus
additional information
ATP, GTP, UTP, CTP show small or no inhibition effects for fraction P3A
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
Oryctolagus cuniculus
-
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
Oryctolagus cuniculus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
isolation of three subcellular fractions: P3A, P3B and P3D, using NaF, EDTA and mercaptoethanol to preserve the activity
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cerebral cortex
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
-
486104
Oryctolagus cuniculus
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
486104
Oryctolagus cuniculus
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
47.8% activity in absence of both EDTA and mercaptoethanol
Oryctolagus cuniculus
mercaptoethanol
47.8% activity in absence of both EDTA and mercaptoethanol
Oryctolagus cuniculus
additional information
activity increases in 2.4fold by preincubation with ATP, MgCl2 and high speed supernatant fractions
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
29% decline in subcellular fraction P3A activity, 43% decline in subcellular fraction P3B activity and 7% decline in subcellular fraction P3D activity
Oryctolagus cuniculus
Alkaline phosphatase
92-95% activity lost for subcellular fractions P3A and P3B
Oryctolagus cuniculus
ATP
little effect on the enzyme activity for the subcellular fraction P3A, 55-64% decline in the subcellular fraction P3B
Oryctolagus cuniculus
CaCl2
1 mM CaCl2 inhibits 73% and 24% activity of subcellular fractions P3B and P3D respectively
Oryctolagus cuniculus
EDTA
inhibits 30% fraction P3D, does not inhibit subcellular fraction P3A
Oryctolagus cuniculus
MgADP-
inhibitory effects less severe than MgATP
Oryctolagus cuniculus
MgAMP
less inhibitory effect than MgADP
Oryctolagus cuniculus
MgATP2-
extreme inhibition in all microsomal fractions
Oryctolagus cuniculus
MgCl2
at 2 mM: 30, 28 and 58% inhibition of subcellular fractions P3A, P3B and P3D activities respectively
Oryctolagus cuniculus
additional information
ATP, GTP, UTP, CTP show small or no inhibition effects for fraction P3A
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
Oryctolagus cuniculus
-
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
Commentary
Organism
isolation of three subcellular fractions: P3A, P3B and P3D, using NaF, EDTA and mercaptoethanol to preserve the activity
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cerebral cortex
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
-
486104
Oryctolagus cuniculus
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
486104
Oryctolagus cuniculus
?
Other publictions for EC 2.3.1.105
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
660411
Nomikos
Application of a TCA-precipita ...
Homo sapiens
Prostaglandins
73
123-140
2004
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1
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1
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1
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1
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1
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1
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1
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1
-
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1
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486104
Baker
MgATP inhibits the synthesis o ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
1213
27-33
1994
3
-
-
-
-
-
10
-
1
-
-
1
-
1
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1
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1
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1
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3
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10
-
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1
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1
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1
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1
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1
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486105
Baker
The potential for platelet-act ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
1170
157-164
1993
1
-
-
-
-
-
2
2
2
1
-
1
-
1
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1
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2
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1
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2
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1
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2
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2
2
1
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1
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2
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1
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2
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486103
Lee
A new de novo pathway for the ...
Rattus norvegicus
J. Biol. Chem.
261
5373-5377
1986
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1
1
1
1
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1
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2
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7
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2
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1
1
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1
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1
1
1
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1
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7
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2
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1
1
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