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Literature summary for 2.3.1.101 extracted from

  • Mamat, B.; Roth, A.; Grimm, C.; Ermler, U.; Tziatzios, C.; Schubert, D.; Thauer, R.K., Shima, S.
    Crystal structures and enzymatic properties of three formyltransferases from archaea: environmental adaptation and evolutionary relationship (2002), Protein Sci., 11, :2168-2178.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.101

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina barkeri
expression in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method at 4°C, crystal structure at 1.9 A resolution Methanosarcina barkeri
vapor diffusion method at 4°C, crystal structure at 2.0 A resolution Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
K3PO4 0.01 M salt concentration is required for 50% activity at 37°C. 0.1 M salt concentration is required for 100% activity at 37°C Methanosarcina barkeri
K3PO4 0.1 M salt concentration is required for 50% activity at 83°C. 1.0 M salt concentration is required for 100% activity at 83°C Archaeoglobus fulgidus
K3PO4 1.0 M salt concentration is required for 50% activity at 89°C. 1.5 M salt concentration is required for 100% activity at 89°C Methanopyrus kandleri

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28076
-
-
Methanopyrus kandleri Q49610
-
-
Methanopyrus kandleri DSM 6324 Q49610
-
-
Methanosarcina barkeri P55301
-
-
Methanosarcina barkeri DSM 804 P55301
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 r
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 r
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 Archaeoglobus fulgidus formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 r
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri DSM 6324 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 r
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri DSM 804 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

Subunits Comment Organism
dimer dimer and tetramer are about 20% and about 80%, respectively, in 20–100 mM potassium phosphate at pH 7.2. The ratio is independent of the buffer concentration. Oligomers larger than a tetramer can not be detected Methanosarcina barkeri
monomer monomer/dimer/tetramer equilibrium. Mostly in a monomeric state at a phosphate concentration of 100 mM and which tetramerizes only potassium phosphate concentrations above 400 mM Methanopyrus kandleri
tetramer dimer and tetramer are about 20% and about 80%, respectively, in 20–100 mM potassium phosphate at pH 7.2. The ratio is independent of the buffer concentration. Oligomers larger than a tetramer can not be detected Methanosarcina barkeri
tetramer the enzyme already reveals a tetrameric state at salt concentrations below 0.1 M Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 50% inactivation after 20 min at 0.005 M K3PO4. No inactivation after 20 min at 0.05 M K3PO4 Archaeoglobus fulgidus
80
-
 50% inactivation after 20 min at 0.05 M K3PO4. No inactivation after 20 min at 0.5 M K3PO4 Methanosarcina barkeri
80
-
 50% inactivation after 20 min at 1.2 M K3PO4. No inactivation after 20 min at 1.5 M K3PO4 Methanopyrus kandleri