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Literature summary for 2.3.1.101 extracted from
- Formylmethanofuran:tetrahydromethanopterin formyltransferase (Ftr) from the hyperthermophilic Methanopyrus kandleri. Cloning, sequencing and functional expression of the ftr gene and one-step purification of the enzyme overproduced in Escherichia coli (1995), Eur. J. Biochem., 230, 906-913.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanopyrus kandleri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
formylmethanofuran | recombinant enzyme | Methanopyrus kandleri |  |
| 0.05 | - |
formylmethanofuran | wild-type enzyme | Methanopyrus kandleri | |
| 0.1 | - |
5,6,7,8-tetrahydromethanopterin | wild-type enzyme | Methanopyrus kandleri | |
| 0.107 | - |
5,6,7,8-tetrahydromethanopterin | recombinant enzyme | Methanopyrus kandleri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| salts | optimal salt concentration for activity: 1.5-2.0 M | Methanopyrus kandleri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
1 * 35000, SDS-PAGE | Methanopyrus kandleri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanopyrus kandleri | Q49610 | strain DSM 6324 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Methanopyrus kandleri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanopyrus kandleri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Methanopyrus kandleri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
recombinant and wild-type enzyme | Methanopyrus kandleri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
stable up to, in presence of salts | Methanopyrus kandleri |
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