General Stability | Organism |
---|---|
presence of salts, 1.5 M, required for optimal stabilization, order of efficiency in protecting the enzyme from heat inactivation at 90°C: K2HPO4, (NH4)2SO4, KCl, NH4Cl, NaCl, Na2SO4, Na2HPO4 | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
formylmethanofuran | - |
Methanopyrus kandleri | |
0.1 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | |
20 | - |
N-Furfurylformamide | - |
Methanopyrus kandleri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | - |
Methanopyrus kandleri | |
K2HPO4 | no activity in absence, assay mixture contains 2 M | Methanopyrus kandleri | |
salts | absolutely dependent on the presence of phosphate or sulfate salts for activity, efficiency of activation in decreasing order: K2HPO4, (NH4)2SO4, K2SO4, Na2SO4, Na2HPO4, no activation: NaCl, KCl, NH4Cl | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE | Methanopyrus kandleri |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | enzyme completely soluble in 80% ammonium sulfate | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Methanopyrus kandleri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | specific for tetrahydromethanopterin | Methanopyrus kandleri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
N-furfurylformamide + 5,6,7,8-tetrahydromethanopterin | pseudo-substrate | Methanopyrus kandleri | 2-(aminomethyl)furane + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
stable up to, in presence of salts | Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Methanopyrus kandleri |