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Literature summary for 2.3.1.101 extracted from
- Formylmethanofuran:tetrahydromethanopterin formyltransferase and N5,N10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea (1993), Arch. Microbiol., 159, 225-232.
General Stability
| General Stability | Organism |
|---|---|
| salts protect against heat inactivation, order of efficiency: K2HPO4, (NH4)2SO4, KCl, NH4Cl, Na2SO4, Na2HPO4 | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus |  |
| 0.032 | - |
formylmethanofuran | - |
Archaeoglobus fulgidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | can substitute for K2HPO4 in stimulation | Archaeoglobus fulgidus | |
| K2HPO4 | 1.5 M stimulates 3.8fold | Archaeoglobus fulgidus | |
| K2HPO4 | no activity in absence, assay mixture contains 2 M | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000 SDS-PAGE | Archaeoglobus fulgidus |
| 125000 | - |
PAGE | Archaeoglobus fulgidus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | enzyme soluble in 60% ammonium sulfate | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
| Archaeoglobus fulgidus VC-19 / DSM 4304 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| relatively insensitive towards inactivation by molecular oxygen | Archaeoglobus fulgidus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1415 | - |
- |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus VC-19 / DSM 4304 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000 SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
complete thermostability in presence of 0.5 M K2HPO4 | Archaeoglobus fulgidus |
| 90 | - |
stable up to, in presence of salts | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Archaeoglobus fulgidus |
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Release 2023.1 (January 2023)
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