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Literature summary for 2.3.1.1 extracted from

  • Sancho-Vaello, E.; Fernandez-Murga, M.L.; Rubio, V.
    Mechanism of arginine regulation of acetylglutamate synthase, the first enzyme of arginine synthesis (2009), FEBS Lett., 583, 202-206.
    View publication on PubMed
Search for more literature for 2.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
delE283delQ284 decrease of enzyme activity, induced substrate inhibition by acetyl-CoA Pseudomonas aeruginosa
delQ284 increase of enzyme activity, triggered acetyl-CoA inhibition Pseudomonas aeruginosa
ins284A lower enzyme activity, decreased inhibition by arginine Pseudomonas aeruginosa
ins284Ains285A lower enzyme acitivity, decreased inhibition by arginine Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA
-
 Pseudomonas aeruginosa
arginine
-
 Pseudomonas aeruginosa
L-glutamate
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 acetyl-CoA wild-type enzyme, in presence of 2 mM arginine Pseudomonas aeruginosa
0.07
-
 acetyl-CoA mutant ins284Ains285A Pseudomonas aeruginosa
0.09
-
 acetyl-CoA wild-type enzyme Pseudomonas aeruginosa
0.1
-
 acetyl-CoA mutant ins284A Pseudomonas aeruginosa
0.13
-
 acetyl-CoA mutant ins284Ains285A, in presence of 2 mM arginine Pseudomonas aeruginosa
0.14
-
 acetyl-CoA mutant ins284A, in presence of 2 mM arginine Pseudomonas aeruginosa
0.15
-
 acetyl-CoA mutant delQ284, in presence of 2 mM arginine Pseudomonas aeruginosa
0.22
-
 acetyl-CoA mutant delE283delQ284, in presence of 2 mM arginine Pseudomonas aeruginosa
0.24
-
 acetyl-CoA mutant delQ284 Pseudomonas aeruginosa
0.26
-
 acetyl-CoA mutant delE283delQ284 Pseudomonas aeruginosa
2.1
-
 L-glutamate mutant ins284A Pseudomonas aeruginosa
2.2
-
 L-glutamate mutant ins284A, in presence of 2 mM arginine Pseudomonas aeruginosa
2.6
-
 L-glutamate mutant ins284Ains285A Pseudomonas aeruginosa
4.9
-
 L-glutamate mutant ins284Ains285A, in presence of 2 mM arginine Pseudomonas aeruginosa
5.2
-
 L-glutamate wild-type enzyme Pseudomonas aeruginosa
20
-
 L-glutamate mutant delQ284 Pseudomonas aeruginosa
85
-
 L-glutamate wild-type enzyme, in presence of 2 mM arginine Pseudomonas aeruginosa
100
-
 L-glutamate mutant delE283delQ284 Pseudomonas aeruginosa
106
-
 L-glutamate mutant delQ284, in presence of 2 mM arginine Pseudomonas aeruginosa
107
-
 L-glutamate mutant delE283delQ284, in presence of 2 mM arginine Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
 mutant delE283delQ284 Pseudomonas aeruginosa
43.6
-
 mutant ins284Ains285A Pseudomonas aeruginosa
48.8
-
 mutant ins284A Pseudomonas aeruginosa
79.4
-
 wild-type enzyme Pseudomonas aeruginosa
114.9
-
 mutant delQ284 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate
-
 Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
N-acetyl-L-glutamate synthase
-
 Pseudomonas aeruginosa
NAGS
-
 Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6
-
 acetyl-CoA mutant delQ284, in presence of 2 mM arginine Pseudomonas aeruginosa
2.4
-
 acetyl-CoA wild-type enzyme, in presence of 2 mM arginine Pseudomonas aeruginosa
3
-
 acetyl-CoA mutant delE283delQ284 Pseudomonas aeruginosa
7.9
-
 acetyl-CoA mutant delQ284 Pseudomonas aeruginosa
10.1
-
 acetyl-CoA mutant delE283delQ284, in presence of 2 mM arginine Pseudomonas aeruginosa
15.1
-
 acetyl-CoA mutant ins284Ains285A, in presence of 2 mM arginine Pseudomonas aeruginosa
60
-
 L-glutamate mutant ins284A Pseudomonas aeruginosa
67
-
 L-glutamate mutant ins284Ains285A Pseudomonas aeruginosa
72
-
 L-glutamate wild-type enzyme Pseudomonas aeruginosa
78
-
 L-glutamate mutant ins284A, in presence of 2 mM arginine Pseudomonas aeruginosa
152
-
 L-glutamate mutant ins284Ains285A, in presence of 2 mM arginine Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function first enzyme of bacterial and plant arginine biosynthesis, activator of urea cycle in animals Pseudomonas aeruginosa