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Literature summary for 2.3.1.1 extracted from

  • Qu, Q.; Morizono, H.; Shi, D.; Tuchman, M.; Caldovic, L.
    A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase (2007), BMC Biochem., 8, 4.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.1

Application

Application Comment Organism
analysis due to the close phylogenetic relationship and similar biochemical properties of xanthomonad NAGS-K and mammalian NAGS the enzyme from Xanthomonas campestris could become a good model for mammalian NAGS in structural, biochemical and biophysical studies Xanthomonas campestris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas campestris

Inhibitors

Inhibitors Comment Organism Structure
NaCl 200 mM NaCl inhibits the activity by about 22% Xanthomonas campestris
Sodium acetate 100 mM NaCl inhibits the activity by about 22% Xanthomonas campestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 acetyl-CoA 30°C, pH 9.0 Xanthomonas campestris
2.8
-
 L-glutamate 30°C, pH 9.0 Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthomonas campestris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76.04
-
-
 Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate no activity with D-glutamate and L-glutamine Xanthomonas campestris CoA + N-acetyl-L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
NAGS-K bifunctional N-acetylglutamate synthase and kinase Xanthomonas campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Xanthomonas campestris

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 pH 8.5: about 65% of maximal activity, pH 10.0: about 80% of maximal activity Xanthomonas campestris