Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arginine | - |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | strong inhibition | Rattus norvegicus | |
AgNO3 | 0.1 mM, 70-90% inhibition | Rattus norvegicus | |
BaCl2 | 1 mM, 30-50% inhibition | Rattus norvegicus | |
CaCl2 | 1 mM, 30-50% inhibition | Rattus norvegicus | |
CoCl2 | 0.1 mM, 30-50% inhibition | Rattus norvegicus | |
coenzyme A | - |
Rattus norvegicus | |
Cu2+ | CuSO4, 0.1 mM, 70-90% inhibition | Rattus norvegicus | |
EDTA | weak inhibition | Rattus norvegicus | |
FeCl3 | 0.1 mM, 30-50% inhibition | Rattus norvegicus | |
FeSO4 | 0.1 mM, 30-50% inhibition | Rattus norvegicus | |
Hg2+ | Hg(CHCOO)2, 0.1 mM, 70-90% inhibition | Rattus norvegicus | |
K2SO4 | - |
Rattus norvegicus | |
KCl | 100 mM, 30% inhibition, 200 mM, 50% inhibition | Rattus norvegicus | |
L-alpha-Acetoxylglutamate | 2 mM, 17% inhibition | Rattus norvegicus | |
MgCl2 | 1 mM, 30-50% inhibition | Rattus norvegicus | |
MnCl2 | 0.1 mM, 30-50% inhibition | Rattus norvegicus | |
N-acetyl-D-glutamate | 2 mM, 30% inhibition | Rattus norvegicus | |
N-acetyl-DL-alpha-aminoadipate | 2 mM, 78% inhibition | Rattus norvegicus | |
N-acetyl-L-aspartate | 2 mM, 25% inhibition | Rattus norvegicus | |
N-acetyl-L-glutamine | 2 mM, 46% inhibition | Rattus norvegicus | |
N-acetylglutamate | - |
Rattus norvegicus | |
N-benzoyl-L-glutamate | 2 mM, 29% inhibition | Rattus norvegicus | |
N-Butyryl-L-glutamate | 2 mM, 19% inhibition | Rattus norvegicus | |
N-carbamoyl-L-glutamate | 2 mM, 31% inhibition | Rattus norvegicus | |
N-propionyl-L-glutamate | 2 mM, 63% inhibition | Rattus norvegicus | |
Ni(NO3)2 | 0.1 mM, 30-50% inhibition | Rattus norvegicus | |
p-hydroxymercuribenzoate | strong inhibition | Rattus norvegicus | |
Pb(NO3)2 | 0.1 mM, 70-90% inhibition | Rattus norvegicus | |
potassium phosphate | - |
Rattus norvegicus | |
succinate | 2 mM, 21% inhibition | Rattus norvegicus | |
Zn2+ | ZnCl2, 0.1 mM, 70-90% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
acetyl-CoA | - |
Rattus norvegicus | |
3 | - |
L-glutamate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | 300000 | gel filtration, mixture of multiple forms | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephadex G-100 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 100 mM phosphate buffer, pH 7.5, 6 months, 50% loss of activity | Rattus norvegicus |
4°C, 100 mM potassium phosphate buffer, pH 7.5, 24 h, 10% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glycine | - |
Rattus norvegicus | CoA + acetylaminoacetate | - |
? | |
acetyl-CoA + L-glutamate | - |
Rattus norvegicus | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamine | - |
Rattus norvegicus | ? | - |
? | |
benzoyl-CoA + L-glutamate | very low activity | Rattus norvegicus | CoA + N-benzoyl-L-glutamate | - |
? | |
butyryl-CoA + L-glutamate | very low activity | Rattus norvegicus | CoA + N-butyryl-L-glutamate | - |
? | |
propionyl-CoA + L-glutamate | - |
Rattus norvegicus | CoA + N-propionyl-L-glutamate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
liver enzyme, in the absence of arginine | Rattus norvegicus |
8 | - |
in the absence of arginine | Rattus norvegicus |
8.5 | - |
in the presence of arginine | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
acetyl-glutamate | - |
Rattus norvegicus |