Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arginine | - |
Homo sapiens | |
arginine | 0.02 mM, 4.2fold activation | Rattus norvegicus | |
Cationic polypeptides | 4fold activation | Rattus norvegicus | |
Triton X-100 | 0.1%, 4fold activation | Rattus norvegicus |
General Stability | Organism |
---|---|
Triton X-100, 0.1%, stabilizes liver enzyme | Rattus norvegicus |
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme | Escherichia coli |
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme | Homo sapiens |
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme | Rattus norvegicus |
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coenzyme A | 2.5 mM, 50% inhibition | Escherichia coli | |
coenzyme A | 1 mM, 20% inhibition | Rattus norvegicus | |
high ionic strength | - |
Escherichia coli | |
high ionic strength | - |
Rattus norvegicus | |
N-acetylglutamate | - |
Escherichia coli | |
N-acetylglutamate | 2 mM, 88% inhibition | Rattus norvegicus | |
propionyl-CoA | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
acetyl-CoA | - |
Rattus norvegicus | |
3.7 | - |
L-glutamate | - |
Rattus norvegicus | |
4.6 | - |
acetyl-CoA | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
4.7 | - |
L-glutamate | partially purified enzyme | Homo sapiens | |
8.1 | - |
acetyl-CoA | partially purified enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
3 * 57000, SDS-PAGE | Rattus norvegicus |
160000 | - |
gel filtration, sucrose density gradient centrifugation | Rattus norvegicus |
190000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | Rattus norvegicus | - |
CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Salmonella enterica subsp. enterica serovar Typhimurium | enzyme catalyzes the first step in the biosynthesis of arginine | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Escherichia coli | enzyme catalyzes the first step in the biosynthesis of arginine | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Pseudomonas aeruginosa | enzyme catalyzes the first step in the biosynthesis of arginine | CoA + N-acetyl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
small intestine | - |
Homo sapiens | - |
small intestine | mucosa | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000014 | - |
- |
Rattus norvegicus |
0.00013 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
0.00013 | - |
- |
Escherichia coli |
0.000268 | - |
activity in liver of adult | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + DL-2-aminopimelate | - |
Escherichia coli | CoA + 2-acetylaminoheptanedioate | - |
? | |
acetyl-CoA + DL-2-aminopimelate | 5.2% of activity with L-glutamate | Rattus norvegicus | CoA + 2-acetylaminoheptanedioate | - |
? | |
acetyl-CoA + glycine | 2.9% of activity with L-glutamate | Rattus norvegicus | CoA + acetylaminoacetate | - |
? | |
acetyl-CoA + L-2-aminoadipate | - |
Rattus norvegicus | CoA + 2-acetylaminohexanedioate | - |
? | |
acetyl-CoA + L-2-aminoadipate | 1.3% of activity with L-glutamate | Escherichia coli | CoA + 2-acetylaminohexanedioate | - |
? | |
acetyl-CoA + L-glutamate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Escherichia coli | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Homo sapiens | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Rattus norvegicus | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Pseudomonas aeruginosa | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | enzyme catalyzes the first step in the biosynthesis of arginine | Salmonella enterica subsp. enterica serovar Typhimurium | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | enzyme catalyzes the first step in the biosynthesis of arginine | Escherichia coli | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | enzyme catalyzes the first step in the biosynthesis of arginine | Pseudomonas aeruginosa | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamine | 5% of activity with acetyl-CoA | Escherichia coli | ? | - |
? | |
acetyl-CoA + L-glutamine | 6.8% of activity with L-glutamate | Rattus norvegicus | ? | - |
? | |
propionyl-CoA + L-glutamate | 4% of activity with acetyl-CoA | Escherichia coli | CoA + N-propionyl-L-glutamate | - |
? | |
propionyl-CoA + L-glutamate | 4.3% of activity with acetyl-CoA | Rattus norvegicus | CoA + N-propionyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 57000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
liver enzyme, in the absence of arginine | Rattus norvegicus |
8.5 | - |
- |
Rattus norvegicus |
8.5 | - |
liver enzyme, in the presence of arginine | Homo sapiens |
10 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
highest stability | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
propionyl-CoA | - |
Rattus norvegicus |