Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel permeation chromatography | Escherichia coli K-12 |
Storage Stability | Organism |
---|---|
4°C, desalting buffer with 50 mM phosphate, 1 mM thiamine diphosphate and 2 mM MgCl2, pH 8.0, 4 weeks, no significant loss of activity | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(2,3-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
2-bromobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(2-bromophenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
2-chlorobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(2-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
2-fluorobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(2-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
2-fluorobenzaldehyde + oxaloacetate | assay at pH 8 | Escherichia coli K-12 | (1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + 2 CO2 | - |
? | |
2-fluorobenzaldehyde + pyruvate | assay at pH 8 | Escherichia coli K-12 | (1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + CO2 | - |
? | |
2-iodobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-5-(2-iodophenyl)-4-oxopentanoic acid + CO2 | - |
? | |
2-methylbenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-5-(2-methylphenyl)-4-oxopentanoic acid + CO2 | - |
? | |
3,4-dihydroxybenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
3-fluorobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(3-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
3-iodobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-5-(3-iodophenyl)-4-oxopentanoic acid + CO2 | - |
? | |
3-methoxybenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-5-(3-methoxyphenyl)-4-oxopentanoic acid + CO2 | - |
? | |
4-chlorbenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(4-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
4-fluorobenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-(4-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
4-hydroxybenzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-5-(4-hydroxyphenyl)-4-oxopentanoic acid + CO2 | - |
? | |
acetaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-4-oxohexanoic acid + CO2 | - |
? | |
benzaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-4-oxo-5-phenylpentanoic acid + CO2 | - |
? | |
benzaldehyde + oxaloacetate | assay at pH 8 | Escherichia coli K-12 | (1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2 | - |
? | |
benzaldehyde + pyruvate | assay at pH 8 | Escherichia coli K-12 | (1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2 | - |
? | |
cyclohex-1-ene-1-carbaldehyde + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-cyclohex-1-en-1-yl-5-hydroxy-4-oxopentanoic acid + CO2 | - |
? | |
glyoxylate + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | 5-hydroxy-4-oxopentanoic acid + 2 CO2 | - |
? | |
hexanal + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-4-oxodecanoic acid + CO2 | - |
? | |
isochorismate + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (1R,2S,5S,6S)-5-[(1-carboxyethenyl)oxy]-2-(3-carboxypropanoyl)-6-hydroxycyclohex-3-ene-1-carboxylic acid + CO2 | - |
? | |
pyruvate + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | 4-hydroxy-5-oxohexanoic acid + 2 CO2 | - |
? | |
undec-10-enal + 2-oxoglutarate | assay at pH 8 | Escherichia coli K-12 | (5R)-5-hydroxy-4-oxopentadec-14-enoic acid + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase | - |
Escherichia coli K-12 |
MenD | - |
Escherichia coli K-12 |
SHCHC synthase | - |
Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli K-12 |