Literature summary for 2.2.1.7 extracted from

DeColli, A.A.; Zhang, X.; Heflin, K.L.; Jordan, F.; Freel Meyers, C.L.
Active site histidines link conformational dynamics with catalysis on anti-infective target 1-deoxy-D-xylulose 5-phosphate synthase (2019), Biochemistry, 58, 4970-4982 .

Search for more literature for 2.2.1.7

Protein Variants

Protein Variants	Comment	Organism
H299A	the mutant shows reduced activity compared to the wild type enzyme	Escherichia coli
H299N	the mutant shows reduced activity compared to the wild type enzyme	Escherichia coli
H49A	the mutant shows reduced activity compared to the wild type enzyme	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Methylacetylphosphonate	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H299N, at pH 8.0 and 25-28°C	Escherichia coli
0.0033	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H299A, at pH 8.0 and 25-28°C	Escherichia coli
0.0142	-	D-glyceraldehyde 3-phosphate	apparent value, wild type enzyme, at pH 8.0 and 25-28°C	Escherichia coli
0.0162	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H49A, at pH 8.0 and 25-28°C	Escherichia coli
0.029	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H49N, at pH 8.0 and 25-28°C	Escherichia coli
0.0442	-	pyruvate	apparent value, wild type enzyme, at pH 8.0 and 25-28°C	Escherichia coli
1.07	-	pyruvate	apparent value, mutant enzyme H49A, at pH 8.0 and 25-28°C	Escherichia coli
1.08	-	pyruvate	apparent value, mutant enzyme H299N, at pH 8.0 and 25-28°C	Escherichia coli
1.1	-	pyruvate	apparent value, mutant enzyme H299A, at pH 8.0 and 25-28°C	Escherichia coli
1.61	-	pyruvate	apparent value, mutant enzyme H49N, at pH 8.0 and 25-28°C	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate + D-glyceraldehyde 3-phosphate	Escherichia coli	-	1-deoxy-D-xylulose 5-phosphate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + D-glyceraldehyde 3-phosphate	-	Escherichia coli	1-deoxy-D-xylulose 5-phosphate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
1-deoxy-D-xylulose 5-phosphate synthase	-	Escherichia coli
DXP synthase	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.045	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H299A, at pH 8.0 and 25-28°C	Escherichia coli
0.05	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H49N, at pH 8.0 and 25-28°C	Escherichia coli
0.17	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H299N, at pH 8.0 and 25-28°C	Escherichia coli
0.45	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H49A, at pH 8.0 and 25-28°C	Escherichia coli
1.38	-	D-glyceraldehyde 3-phosphate	apparent value, wild type enzyme, at pH 8.0 and 25-28°C	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00324	-	Methylacetylphosphonate	wild type enzyme, at pH 8.0 and 25-28°C	Escherichia coli
0.125	-	Methylacetylphosphonate	mutant enzyme H49A, at pH 8.0 and 25-28°C	Escherichia coli
0.167	-	Methylacetylphosphonate	mutant enzyme H49N, at pH 8.0 and 25-28°C	Escherichia coli
0.24	-	Methylacetylphosphonate	mutant enzyme H299N, at pH 8.0 and 25-28°C	Escherichia coli
1.08	-	Methylacetylphosphonate	mutant enzyme H299A, at pH 8.0 and 25-28°C	Escherichia coli

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.01	-	wild type enzyme, in the presence of 0.08 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
0.0319	-	wild type enzyme, in the presence of 2 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
0.38	-	mutant enzyme H49A, in the presence of 0.08 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
0.52	-	mutant enzyme H49N, in the presence of 0.08 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
0.78	-	mutant enzyme H299N, in the presence of 0.08 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
1.06	-	mutant enzyme H49A, in the presence of 2 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
1.7	-	mutant enzyme H49N, in the presence of 2 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
2.84	-	mutant enzyme H299N, in the presence of 2 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
3.2	-	mutant enzyme H299A, in the presence of 0.08 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate
10	-	mutant enzyme H299A, in the presence of 2 mM pyruvate, at pH 8.0 and 25-28°C	Escherichia coli	Methylacetylphosphonate

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.037	-	pyruvate	apparent value, mutant enzyme H299A, at pH 8.0 and 25-28°C	Escherichia coli
0.173	-	pyruvate	apparent value, mutant enzyme H299N, at pH 8.0 and 25-28°C	Escherichia coli
0.417	-	pyruvate	apparent value, mutant enzyme H49N, at pH 8.0 and 25-28°C	Escherichia coli
0.422	-	pyruvate	apparent value, mutant enzyme H49A, at pH 8.0 and 25-28°C	Escherichia coli
10.8	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H299N, at pH 8.0 and 25-28°C	Escherichia coli
15	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H299A, at pH 8.0 and 25-28°C	Escherichia coli
18.8	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H49N, at pH 8.0 and 25-28°C	Escherichia coli
27.3	-	D-glyceraldehyde 3-phosphate	apparent value, mutant enzyme H49A, at pH 8.0 and 25-28°C	Escherichia coli
32.2	-	pyruvate	apparent value, wild type enzyme, at pH 8.0 and 25-28°C	Escherichia coli
95	-	D-glyceraldehyde 3-phosphate	apparent value, wild type enzyme, at pH 8.0 and 25-28°C	Escherichia coli

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Release 2023.1 (January 2023)