Cloned (Comment) | Organism |
---|---|
- |
Botryococcus braunii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
0.72 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
0.75 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
0.87 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
1 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
1.8 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | sequence contains a plastid targeting peptide | Botryococcus braunii | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, maximal activity at 4-12 mM | Botryococcus braunii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72500 | - |
x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein | Botryococcus braunii |
77400 | - |
x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
77400 | - |
x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
78500 | - |
x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein | Botryococcus braunii |
82300 | - |
x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
83100 | - |
x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botryococcus braunii | H5ZXI0 | isoform DXS-II | - |
Botryococcus braunii | H5ZXI1 | isoform DXS-II | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a plastid targeting peptide | Botryococcus braunii |
Storage Stability | Organism |
---|---|
-20°C, elution buffer containing 30% glycerol, storage for around 10 days with no significant loss of activity | Botryococcus braunii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde 3-phosphate | - |
Botryococcus braunii | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein | Botryococcus braunii |
? | x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
? | x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
Synonyms | Comment | Organism |
---|---|---|
DXS-I | - |
Botryococcus braunii |
DXS-II | - |
Botryococcus braunii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Botryococcus braunii |
34 | - |
- |
Botryococcus braunii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
75% of the activity at 34°C | Botryococcus braunii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
sharp decrease in activity above | Botryococcus braunii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
2 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
2.4 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
5.6 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
6 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Botryococcus braunii |
Organism | Comment | Expression |
---|---|---|
Botryococcus braunii | mRNA accumulation patterns over time are almost identical among the three DXS isoforms reaching the highest expression levels on day 6 after inoculation into fresh medium | additional information |