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Literature summary for 2.2.1.7 extracted from
- Cloning and characterization of the dxs gene, encoding 1-deoxy-d-xylulose 5-phosphate synthase from Agrobacterium tumefaciens, and its overexpression in Agrobacterium tumefaciens (2007), J. Biotechnol., 128, 555-566.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | Agrobacterium tumefaciens strain KCCM 10413, overexpressing the enzyme might be useful in industrial production of ubiquinone-10, i.e. UbiQ(10) | Agrobacterium tumefaciens |
| synthesis | Agrobacterium tumefaciens strain KCCM 10413, overexpressing the enzyme might be useful in industrial production of ubiquinone-10, i.e. UbiQ(10) | Agrobacterium tumefaciens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Agrobacterium tumefaciens |
| gene dxs11, DNA and amino acid sequence determination and analysis, functional overexpression of the soluble enzyme in Escherichia coli, functional overexpression in Agrobacterium tumefaciens strain KCCM 10413, the transformed strain shows increased enzyme activity | Agrobacterium tumefaciens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H48A | mutant protein with lost activity, below 0.1% of specific activity compared with that of the wild-type | Agrobacterium tumefaciens |
| additional information | the dxs11 gene is transformed into Agrobacterium tumefaciens KCCM 10413, and the resulting recombinant, Agrobacterium tumefaciens pGX11, shows higher UbiQ10 production (502.4 mg/l) and content (8.3 mg/g DCW) than Agrobacterium tumefaciens KCCM 10413, by 21.9 and 23.9%, respectively | Agrobacterium tumefaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0232 | - |
D-glyceraldehyde 3-phosphate | - |
Agrobacterium tumefaciens |  |
| 0.04 | - |
pyruvate | - |
Agrobacterium tumefaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme requires a divalent cation | Agrobacterium tumefaciens | |
| Mg2+ | enzyme requires either Mg2+ or Mn2+, maximum activity at 1 mM Mg2+ | Agrobacterium tumefaciens | |
| Mn2+ | the enzyme requires a divalent cation | Agrobacterium tumefaciens | |
| Mn2+ | enzyme requires either Mg2+ or Mn2+, maximum activity at 2 mM Mn2+ | Agrobacterium tumefaciens | |
| thiamine diphosphate | required for enzymatic activity | Agrobacterium tumefaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68050 | - |
SDS-PAGE | Agrobacterium tumefaciens |
| 68054 | - |
x * 68054, sequence calculation | Agrobacterium tumefaciens |
| 135000 | - |
native enzyme, gel filtration | Agrobacterium tumefaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + D-glyceraldehyde 3-phosphate | Agrobacterium tumefaciens | - |
1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
| pyruvate + D-glyceraldehyde 3-phosphate | Agrobacterium tumefaciens KCCM 10413 | - |
1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
gene dxs11 | - |
| Agrobacterium tumefaciens | Q5QKF8 | - |
- |
| Agrobacterium tumefaciens KCCM 10413 | - |
gene dxs11 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| functional soluble, recombinant enzyme from Escherichia coli | Agrobacterium tumefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Agrobacterium tumefaciens |
| 11.2 | - |
wild-type | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + D-glyceraldehyde 3-phosphate | - |
Agrobacterium tumefaciens | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
| pyruvate + D-glyceraldehyde 3-phosphate | - |
Agrobacterium tumefaciens KCCM 10413 | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68054, sequence calculation | Agrobacterium tumefaciens |
| homodimer | gel filtration | Agrobacterium tumefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-deoxy-D-xylulose 5-phosphate synthase 2 | - |
Agrobacterium tumefaciens |
| DXS | - |
Agrobacterium tumefaciens |
| dxs11 | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Agrobacterium tumefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
preparations are incubated at 4, 20, 30, 45, and 50°C, and retain 50% of their initial activities after 90 days, 7 days, 3 days, 2.5 h and 2 min, respectively | Agrobacterium tumefaciens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.8 | - |
pyruvate | - |
Agrobacterium tumefaciens | |
| 26.8 | - |
pyruvate | pH 8.0, 37°C | Agrobacterium tumefaciens | |
| 26.8 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 37°C | Agrobacterium tumefaciens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Agrobacterium tumefaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | required | Agrobacterium tumefaciens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Agrobacterium tumefaciens | amino acid sequence calculation | - |
5.63 |
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