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Literature summary for 2.2.1.6 extracted from
- Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families (2017), Proc. Natl. Acad. Sci. USA, 114, E1091-E1100 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with propoxycarbazone or thiencarbazone methyl, hanging drop vapor diffusion method, using 1.0 M Na/K tartrate, 0.1 M CHES, and 0.19 M (NH4)2SO4. In complex with bispyribac or pyrithiobac, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M sodium citrate tribasic dihydrate or 0.2 M potassium citrate tribasic dihydrate | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bispyribac | about 58% enzyme inhibition after 2 h at enzyme:inhibitor ratio of 10:1 | Arabidopsis thaliana |  |
| chlorimuron ethyl | about 90% enzyme inhibition after 2 h at enzyme:inhibitor ratio of 10:1 | Arabidopsis thaliana | |
| imazaquin | about 20% enzyme inhibition after 2 h at enzyme:inhibitor ratio of 10:1, about 85% enzyme inhibition after 2 days at enzyme:inhibitor ratio of 1000:1 | Arabidopsis thaliana | |
| penoxsulam | - |
Arabidopsis thaliana | |
| propoxycarbazone | about 55% enzyme inhibition after 2 h at enzyme:inhibitor ratio of 10:1 | Arabidopsis thaliana | |
| pyrithiobac | about 70% enzyme inhibition after 2 h at enzyme:inhibitor ratio of 10:1 | Arabidopsis thaliana | |
| thiencarbazone methyl | about 40% enzyme inhibition after 2 h at enzyme:inhibitor ratio of 10:1 | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 pyruvate | Arabidopsis thaliana | - |
2-acetolactate + CO2 | - |
? | |
| pyruvate + 2-oxobutyrate | Arabidopsis thaliana | - |
2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P17597 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography and S-200 HR gel filtration | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 pyruvate | - |
Arabidopsis thaliana | 2-acetolactate + CO2 | - |
? | |
| pyruvate + 2-oxobutyrate | - |
Arabidopsis thaliana | 2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetohydroxyacid synthase | - |
Arabidopsis thaliana |
| AHAS | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Arabidopsis thaliana | |
| thiamine diphosphate | - |
Arabidopsis thaliana | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000185 | - |
imazaquin | at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 0.0000409 | - |
bispyribac | at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 0.0000747 | - |
chlorimuron ethyl | at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 0.000179 | - |
propoxycarbazone | at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 0.0004348 | - |
pyrithiobac | at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 0.0006708 | - |
thiencarbazone methyl | at pH 7.2 and 30°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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