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Literature summary for 2.2.1.6 extracted from

  • Yang, Q.; Deng, W.; Wang, S.; Liu, H.; Li, X.; Zheng, M.
    Effects of resistance mutations of Pro197, Asp376 and Trp574 on the characteristics of acetohydroxyacid synthase (AHAS) isozymes (2018), Pest Manag. Sci., 74, 1870-1879 .
    View publication on PubMed
Search for more literature for 2.2.1.6

Protein Variants

Protein Variants Comment Organism
D376E the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
P197H the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
P197L the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
P197T the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
W574L the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia

Inhibitors

Inhibitors Comment Organism Structure
L-isoleucine
-
 Descurainia sophia
L-leucine
-
 Descurainia sophia
L-valine
-
 Descurainia sophia
tribenuron-methyl
-
 Descurainia sophia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.09
-
 pyruvate mutant enzyme W574L, at pH 7.5 and 37°C Descurainia sophia
1.19
-
 pyruvate mutant enzyme P197T, at pH 7.5 and 37°C Descurainia sophia
1.37
-
 pyruvate mutant enzyme P197L, at pH 7.5 and 37°C Descurainia sophia
1.38
-
 pyruvate mutant enzyme P197H, at pH 7.5 and 37°C Descurainia sophia
1.46
-
 pyruvate mutant enzyme P197S, at pH 7.5 and 37°C Descurainia sophia
1.5 2 pyruvate mutant enzyme D376E, at pH 7.5 and 37°C Descurainia sophia
1.74
-
 pyruvate wild type enzyme, at pH 7.5 and 37°C Descurainia sophia

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.5 mM used in assay conditions Descurainia sophia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Descurainia sophia
-
 2-acetolactate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Descurainia sophia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephadex G-25 gel filtration Descurainia sophia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Descurainia sophia 2-acetolactate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
acetohydroxyacid synthase
-
 Descurainia sophia
acetolactate synthase
-
 Descurainia sophia
AHAS
-
 Descurainia sophia
ALS1
-
 Descurainia sophia

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Descurainia sophia
thiamine diphosphate
-
 Descurainia sophia