Cloned (Comment) | Organism |
---|---|
genes MGG_01104 and MGG_06868, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of GFP-tagged subunits in the deletion mutant strains, subcellular localization of both MoIlv2 and MoIlv6 to the mitochondria | Pyricularia oryzae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of the deletion mutants DELTAMoilv2 and DELTAMoilv6. Phenotypic analysis shows that both mutants are auxotrophic for leucine, isoleucine and valine, and are defective in conidial morphogenesis, appressorial penetration and pathogenicity | Pyricularia oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Pyricularia oryzae | 5739 | - |
mitochondrion | MoIlv2 and MoIlv6 are both localized to the mitochondria and the signal peptide of MoIlv6 is critical for its localization | Pyricularia oryzae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | Pyricularia oryzae | - |
2-acetolactate + CO2 | - |
? | |
2 pyruvate | Pyricularia oryzae Guy11 | - |
2-acetolactate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyricularia oryzae | - |
- |
- |
Pyricularia oryzae | G4NC97 and G4MMK2 | small subunit Moilv6 and large subunit Moilv2; genes ilv2 and ilv6 encoding the catalytic and the regulatory subunit, respectively | - |
Pyricularia oryzae Guy11 | - |
- |
- |
Pyricularia oryzae Guy11 | G4NC97 and G4MMK2 | small subunit Moilv6 and large subunit Moilv2; genes ilv2 and ilv6 encoding the catalytic and the regulatory subunit, respectively | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Pyricularia oryzae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Pyricularia oryzae | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Pyricularia oryzae Guy11 | 2-acetolactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme has a catalytic subunit and a regulatory subunit, encoded by two different genes | Pyricularia oryzae |
Synonyms | Comment | Organism |
---|---|---|
ALS | - |
Pyricularia oryzae |
Ilv2 | isoform | Pyricularia oryzae |
Ilv6 | isoform | Pyricularia oryzae |
Moilv2 | - |
Pyricularia oryzae |
Moilv6 | - |
Pyricularia oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pyricularia oryzae |
General Information | Comment | Organism |
---|---|---|
malfunction | the deletion mutants DELTAMoilv2 and DELTAMoilv6 are both auxotrophic for leucine, isoleucine and valine, and are defective in conidial morphogenesis, appressorial penetration and pathogenicity, phenotypes, overview | Pyricularia oryzae |
metabolism | acetolactate synthase catalyses the first common step in leucine, isoleucine and valine biosynthesis | Pyricularia oryzae |
metabolism | isoform Ilv2 plays a crucial role in isoleucine and valine biosynthesis, isoform Ilv6 contributes to isoleucine and leucine biosynthesis | Pyricularia oryzae |
additional information | the domains are crucial to the function of MoIlv2 and MoIlv6 during pathogenicity on rice and barley leaves, functional analysis of enzyme domains, overview | Pyricularia oryzae |
physiological function | MoIlv2 plays a crucial role in isoleucine and valine biosynthesis, whereas MoIlv6 contributes to isoleucine and leucine biosynthesis, both genes are required for fungal pathogenicity. MoIlv2 and MoIlv6 play a critical role in maintaining the balance of intracellular amino acid levels. And MoIlv2 and MoIlv6 are involved in aerial hyphal growth, pigmentation, conidial morphogenesis and pathogenicity on rice and barley | Pyricularia oryzae |
physiological function | the enzyme plays a critical role in maintaining the balance of intracellular amino acid level, is involved in aerial hyphal growth, pigmentation, conidial morphogenesis, and is required for fungal pathogenicity | Pyricularia oryzae |