Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Azoarcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | - |
- |
- |
Azoarcus sp. 22Lin | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate | Azoarcus sp. | (S)-acetoin + 2 CO2 | 87-90%ee | ? | |
2 pyruvate | enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate | Azoarcus sp. 22Lin | (S)-acetoin + 2 CO2 | 87-90%ee | ? | |
additional information | ThDP-dependent cyclohexane-1,2-dione hydrolase (CDH) is able to form (S)-acetoin with particularly high enantioselectivity (up to 95%ee) by all three possible pathways: homocoupling of pyruvate, homocoupling of acetaldehyde, or cross-coupling of acetaldehyde (as acceptor) and pyruvate (as donor), high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin. An unprecedented non-acetolactate pathway for the homocoupling of pyruvate explains the high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin, enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate, competition assay, mechanism, overview | Azoarcus sp. | ? | - |
? | |
additional information | ThDP-dependent cyclohexane-1,2-dione hydrolase (CDH) is able to form (S)-acetoin with particularly high enantioselectivity (up to 95%ee) by all three possible pathways: homocoupling of pyruvate, homocoupling of acetaldehyde, or cross-coupling of acetaldehyde (as acceptor) and pyruvate (as donor), high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin. An unprecedented non-acetolactate pathway for the homocoupling of pyruvate explains the high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin, enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate, competition assay, mechanism, overview | Azoarcus sp. 22Lin | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Azoarcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Azoarcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one molecule per enzyme molecule | Azoarcus sp. | |
thiamine diphosphate | dependent on, one molecule per enzyme molecule | Azoarcus sp. |