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Literature summary for 2.2.1.6 extracted from

  • Singh, V.; Chandra, D.; Srivastava, B.S.; Srivastava, R.
    Biochemical and transcription analysis of acetohydroxyacid synthase isoforms in Mycobacterium tuberculosis identifies these enzymes as potential targets for drug development (2011), Microbiology, 157, 29-37.
    View publication on PubMed
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.9
-
 pyruvate pH 7.5, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Ks value 0.183 mM Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O06335 catalytic subunit ilvB2
-
Mycobacterium tuberculosis O53554 catalytic subunit ilvX
-
Mycobacterium tuberculosis P9WG39 catalytic subunit ilvG
-
Mycobacterium tuberculosis P9WG41 catalytic subunit ilvB1
-
Mycobacterium tuberculosis H37Rv P9WG39 catalytic subunit ilvG
-
Mycobacterium tuberculosis H37Rv P9WG41 catalytic subunit ilvB1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
 pH 7.5, 37°C Mycobacterium tuberculosis
11
-
 presence of 20 mM acetate, pH 7.5, 37°C Mycobacterium tuberculosis
36
-
 presence of 20 mM acetate, pH 7.5, 37°C Mycobacterium tuberculosis
42
-
 pH 7.5, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2 pyruvate catalytic subunit ilvG shows positive cooperativity towards substrate and cofactors Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2 pyruvate catalytic subunit ilvG shows positive cooperativity towards substrate and cofactors Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?
2 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ilvB1
-
 Mycobacterium tuberculosis
ilvB2
-
 Mycobacterium tuberculosis
ilvG
-
 Mycobacterium tuberculosis
ilvX
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate Ks value 0.042 mM Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis expression level is 0.16 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in vitro additional information
Mycobacterium tuberculosis expression level is 0.53 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in culture additional information
Mycobacterium tuberculosis expression level is 0.6 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in vitro additional information
Mycobacterium tuberculosis expression level is 0.63 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in vitro additional information
Mycobacterium tuberculosis expression is upregulated in extended stationary phase, ex vivo, and in acid stress and hypoxic environments up