Protein Variants | Comment | Organism |
---|---|---|
F147A | 4fold decrease in vmax value, strong resistance to sulfonylurea inhibitors | Mycobacterium tuberculosis |
F147R | 2.5fold decrease in vmax value, strong resistance to sulfonylurea inhibitors | Mycobacterium tuberculosis |
L141A | 5fold decrease in vmax value | Mycobacterium tuberculosis |
W561R | 30fold decrease in vmax value, strong resistance to sulfonylurea inhibitors | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-isoleucine | - |
Mycobacterium tuberculosis | |
L-leucine | - |
Mycobacterium tuberculosis | |
L-valine | - |
Mycobacterium tuberculosis | |
Londax | - |
Mycobacterium tuberculosis | |
NC-311 | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
pyruvate | mutant F147R, pH 7.5, 37°C | Mycobacterium tuberculosis | |
1.18 | - |
pyruvate | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
1.89 | - |
pyruvate | mutant L141A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
2.49 | - |
pyruvate | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
3.35 | - |
pyruvate | mutant L89A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
3.89 | - |
pyruvate | mutant F147A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
4.94 | - |
pyruvate | mutant W516R, pH 7.5, 37°C | Mycobacterium tuberculosis | |
5.8 | - |
pyruvate | mutant R101A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
8.9 | - |
pyruvate | mutant S27A, pH 7.5, 37°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | Mycobacterium tuberculosis |
68000 | - |
x * 68000, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
regulatory subunit | - |
Mycobacterium tuberculosis | P9WG41 | catalytic subunit | - |
Mycobacterium tuberculosis H37Rv | P9WG41 | catalytic subunit | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.17 | - |
isolated catalytic subunit, pH 7.5, 37°C | Mycobacterium tuberculosis |
1.13 | - |
pH 7.5, 37°C | Mycobacterium tuberculosis |
1.13 | - |
holoenzyme, pH 7.5, 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Mycobacterium tuberculosis | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Mycobacterium tuberculosis H37Rv | 2-acetolactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Mycobacterium tuberculosis |
? | x * 68000, SDS-PAGE | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | Km value 0.027 mM | Mycobacterium tuberculosis | |
thiamine diphosphate | Km value 0.036 mM | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00064 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | Londax | |
0.0014 | - |
R101A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-leucine | |
0.0016 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | L-leucine | |
0.004 | - |
R101A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-valine | |
0.005 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | L-valine | |
0.0075 | - |
mutant S27A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-valine | |
0.011 | - |
mutant L141A, pH 7.5, 37°C | Mycobacterium tuberculosis | NC-311 | |
0.012 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | L-isoleucine | |
0.012 | - |
L89A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-valine | |
0.021 | - |
mutant L141A, pH 7.5, 37°C | Mycobacterium tuberculosis | Londax | |
0.04 | - |
R101A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-isoleucine | |
0.048 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | NC-311 | |
0.062 | - |
mutant S27A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-isoleucine | |
0.32 | - |
L89A, pH 7.5, 37°C | Mycobacterium tuberculosis | L-isoleucine |