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Literature summary for 2.2.1.6 extracted from

  • Pham, N.C.; Moon, J.Y.; Cho, J.H.; Lee, S.J.; Park, J.S.; Kim, D.E.; Park, Y.; Yoon, M.Y.
    Characterization of acetohydroxyacid synthase I from Escherichia coli K-12 and identification of its inhibitors (2010), Biosci. Biotechnol. Biochem., 74, 2281-2286.
    View publication on PubMed
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1-(4,6-dimethoxypyrimidin-2-yl)-5-methoxymethyl-N-(2-isopropyl-6-nitrophenyl)-1H-1,2,4-triazole-3-sulfonamide
-
 Escherichia coli
1-(4,6-dimethoxypyrimidin-2-yl)-5-methyl-N-(2-isopropyl-6-nitrophenyl)-1H-1,2,4-triazole-3-sulfonamide
-
 Escherichia coli
1-(4,6-dimethoxypyrimidin-2-yl)-5-methylthio-N-(2-chloro-6-fluorophenyl)-1H-1,2,4-triazole-3-sulfonamide
-
 Escherichia coli
1-(4-chloro-6-methoxypyrimidin-2-yl)-5-methoxy-N-(2-methyl-6-nitrophenyl)-1H-1,2,4-triazole-3-sulfonamide
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.15
-
 pyruvate pH 7.6, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km value 1.26 mM Escherichia coli
additional information a divalent ion is required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 isoform acetohydroxyacid synthase I
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
 pH 7.6, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Escherichia coli 2-acetolactate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
ilvB
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD Km value 0.061 microM Escherichia coli
thiamine diphosphate Km value 0.2 mM Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.021
-
 pH 7.6, 37°C Escherichia coli 1-(4,6-dimethoxypyrimidin-2-yl)-5-methoxymethyl-N-(2-isopropyl-6-nitrophenyl)-1H-1,2,4-triazole-3-sulfonamide
0.022
-
 pH 7.6, 37°C Escherichia coli 1-(4,6-dimethoxypyrimidin-2-yl)-5-methyl-N-(2-isopropyl-6-nitrophenyl)-1H-1,2,4-triazole-3-sulfonamide
0.03
-
 pH 7.6, 37°C Escherichia coli 1-(4-chloro-6-methoxypyrimidin-2-yl)-5-methoxy-N-(2-methyl-6-nitrophenyl)-1H-1,2,4-triazole-3-sulfonamide
0.032
-
 pH 7.6, 37°C Escherichia coli 1-(4,6-dimethoxypyrimidin-2-yl)-5-methylthio-N-(2-chloro-6-fluorophenyl)-1H-1,2,4-triazole-3-sulfonamide