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Literature summary for 2.2.1.6 extracted from

  • Yoon, M.; Gedi, V.; Kim, J.; Park, Y.; Kim, D.; Park, E.; Choi, J.
    Structural and functional evaluation of three well-conserved serine residues in tobacco acetohydroxyacid synthase (2010), Biochimie, 92, 65-70.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of enzyme based on the structure of the yeast enzyme.In the model, the S167 and S506 residues lie near the FAD binding site, while the S539 residue is found near the thiamine diphosphate binding site Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
S167A 73% of wild-type activity Nicotiana tabacum
S167F inactive, mutation abolishes the binding affinity for cofactor FAD. The far-UV spectrum is similar to wild-type Nicotiana tabacum
S167R 250% of wild-type activity Nicotiana tabacum
S506A 230% of wild-type activity Nicotiana tabacum
S506F inactive, mutation abolishes the binding affinity for cofactor FAD. The far-UV spectrum is similar to wild-type Nicotiana tabacum
S506R 183% of wild-type activity Nicotiana tabacum
S539A 73% of wild-type activity, strong resistance to herbicides NC-311, a sulfonylurea, Cadre, an imidazolinone, and a triazolopyrimidine Nicotiana tabacum
S539F 171% of wild-type activity, strong resistance to herbicides NC-311, a sulfonylurea, Cadre, an imidazolinone, and a triazolopyrimidine Nicotiana tabacum
S539R 30% of wild-type activity Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
Cadre imidazolinone inhibitor Nicotiana tabacum
NC-311 sulfonylurea inhibitor Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.38
-
pyruvate mutant S539R, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
3.33
-
pyruvate mutant S539A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
3.36
-
pyruvate mutant S167A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
4.58
-
pyruvate wild-type, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
7.82
-
pyruvate mutant S539F, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
8.36
-
pyruvate mutant S506R, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
10.54
-
pyruvate mutant S506A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum
11.48
-
pyruvate mutant S167R, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
Nicotiana tabacum 2-acetolactate + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Nicotiana tabacum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00024
-
mutant S167R, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum Cadre
0.00089
-
mutant S506A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum Cadre
0.0026
-
mutant S167A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum Cadre
0.0035
-
wild-type, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum Cadre
0.008
-
mutant S506A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum NC-311
0.032
-
mutant S167A, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum NC-311
0.054
-
wild-type, pH not specified in the publication, temperature not specified in the publication Nicotiana tabacum NC-311