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Literature summary for 2.2.1.6 extracted from

  • Shin, H.J.; Chong, C.K.; Chang, S.I.; Choi, J.D.
    Structural and functional role of cysteinyl residues in tobacco acetolactate synthase (2000), Biochem. Biophys. Res. Commun., 271, 801-806.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C163S labile, readlily degraded Nicotiana tabacum
C309S labile, readlily degraded Nicotiana tabacum
C411A no enzymic activity, no binding of FAD Nicotiana tabacum
C607S no significant effects Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one wild-type, 50% inhibition at 0.00318 mM, mutant C411S, 50% inhibition at 0.00426 mM, mutant C607S, 50% inhibition at 0.00099 mM Nicotiana tabacum
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide wild-type, 50% inhibition at 0.00262 mM, mutant C411S, 50% inhibition at 0.00668 mM, mutant C607S, 50% inhibition at 0.00887 mM Nicotiana tabacum
NC-311 sulfonylurea derivative, wild-type, 50% inhibition at 9.39 nM, mutant C411S, 50% inhibition at 16.83 nM, mutant C607S, 50% inhibition at 20.25 nM Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.68
-
pyruvate mutant C607S, pH 7.5, 37°C Nicotiana tabacum
15.05
-
pyruvate wild-type, pH 7.5, 37°C Nicotiana tabacum
21.55
-
pyruvate mutant C411S, pH 7.5, 37°C Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification disulfide bond between C163 and C309 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Nicotiana tabacum 2-acetolactate + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Nicotiana tabacum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000939
-
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide wild-type, pH 7.5, 37°C Nicotiana tabacum
0.00000939
-
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one wild-type, pH 7.5, 37°C Nicotiana tabacum
0.00000939
-
NC-311 wild-type, pH 7.5, 37°C Nicotiana tabacum
0.0000168
-
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant C411S, pH 7.5, 37°C Nicotiana tabacum
0.0000168
-
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant C411S, pH 7.5, 37°C Nicotiana tabacum
0.0000168
-
NC-311 mutant C411S, pH 7.5, 37°C Nicotiana tabacum
0.0000202
-
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant C607S, pH 7.5, 37°C Nicotiana tabacum
0.0000202
-
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant C607S, pH 7.5, 37°C Nicotiana tabacum
0.0000202
-
NC-311 mutant C607S, pH 7.5, 37°C Nicotiana tabacum