Protein Variants | Comment | Organism |
---|---|---|
C163S | labile, readlily degraded | Nicotiana tabacum |
C309S | labile, readlily degraded | Nicotiana tabacum |
C411A | no enzymic activity, no binding of FAD | Nicotiana tabacum |
C607S | no significant effects | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | wild-type, 50% inhibition at 0.00318 mM, mutant C411S, 50% inhibition at 0.00426 mM, mutant C607S, 50% inhibition at 0.00099 mM | Nicotiana tabacum | |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | wild-type, 50% inhibition at 0.00262 mM, mutant C411S, 50% inhibition at 0.00668 mM, mutant C607S, 50% inhibition at 0.00887 mM | Nicotiana tabacum | |
NC-311 | sulfonylurea derivative, wild-type, 50% inhibition at 9.39 nM, mutant C411S, 50% inhibition at 16.83 nM, mutant C607S, 50% inhibition at 20.25 nM | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.68 | - |
pyruvate | mutant C607S, pH 7.5, 37°C | Nicotiana tabacum | |
15.05 | - |
pyruvate | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
21.55 | - |
pyruvate | mutant C411S, pH 7.5, 37°C | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | disulfide bond between C163 and C309 | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000939 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.00000939 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.00000939 | - |
NC-311 | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.0000168 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant C411S, pH 7.5, 37°C | Nicotiana tabacum | |
0.0000168 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant C411S, pH 7.5, 37°C | Nicotiana tabacum | |
0.0000168 | - |
NC-311 | mutant C411S, pH 7.5, 37°C | Nicotiana tabacum | |
0.0000202 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant C607S, pH 7.5, 37°C | Nicotiana tabacum | |
0.0000202 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant C607S, pH 7.5, 37°C | Nicotiana tabacum | |
0.0000202 | - |
NC-311 | mutant C607S, pH 7.5, 37°C | Nicotiana tabacum |