Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 200 mM, 1.6fold stimulation | Methanococcus aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
pyruvate | - |
Methanococcus aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ or Mg2+ required | Methanococcus aeolicus | |
Mg2+ | Km: 0.3 mM MgCl2 | Methanococcus aeolicus | |
Mn2+ | Mn2+ or Mg2+ required | Methanococcus aeolicus | |
Mn2+ | Km: 0.016 mM MnCl2 | Methanococcus aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus aeolicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
5% O2, half-life: 35 min | Methanococcus aeolicus |
Purification (Comment) | Organism |
---|---|
- |
Methanococcus aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Methanococcus aeolicus | 2-acetolactate + CO2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Methanococcus aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | Km: 0.0004 mM | Methanococcus aeolicus | |
FMN | stimulates, Km: 0.0013 mM | Methanococcus aeolicus | |
riboflavin | plus 10 mM phosphate, stimulates | Methanococcus aeolicus | |
thiamine diphosphate | required as cofactor | Methanococcus aeolicus | |
thiamine diphosphate | Km: 0.0016 | Methanococcus aeolicus |