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Literature summary for 2.2.1.6 extracted from

  • Xing, R.; Whitman, W.B.
    Purification and characterization of the oxygen-sensitive acetohydroxy acid synthase from the archaebacterium Methanococcus aeolicus (1994), J. Bacteriol., 176, 1207-1213.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 200 mM, 1.6fold stimulation Methanococcus aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.8
-
pyruvate
-
Methanococcus aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ or Mg2+ required Methanococcus aeolicus
Mg2+ Km: 0.3 mM MgCl2 Methanococcus aeolicus
Mn2+ Mn2+ or Mg2+ required Methanococcus aeolicus
Mn2+ Km: 0.016 mM MnCl2 Methanococcus aeolicus

Organism

Organism UniProt Comment Textmining
Methanococcus aeolicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
5% O2, half-life: 35 min Methanococcus aeolicus

Purification (Commentary)

Purification (Comment) Organism
-
Methanococcus aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Methanococcus aeolicus 2-acetolactate + CO2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Methanococcus aeolicus

Cofactor

Cofactor Comment Organism Structure
FAD Km: 0.0004 mM Methanococcus aeolicus
FMN stimulates, Km: 0.0013 mM Methanococcus aeolicus
riboflavin plus 10 mM phosphate, stimulates Methanococcus aeolicus
thiamine diphosphate required as cofactor Methanococcus aeolicus
thiamine diphosphate Km: 0.0016 Methanococcus aeolicus