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Literature summary for 2.2.1.6 extracted from

  • Chang, S.I.; Kang, M.K.; Choi, J.D.; Namgoong, S.K.
    Soluble overexpression in Escherichia coli, and purification and characterization of wild-type recombinant tobacco acetolactate synthase (1997), Biochem. Biophys. Res. Commun., 234, 549-553.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the bacterial expression vector pGEX-2T and used to transform Escherichia coli strain pGEX-2T Nicotiana tabacum
wild-type recombinant enzyme fused with glutathione S-transferase Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
metsulfuron-methyl
-
Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.1
-
pyruvate wild-type recombinant enzyme Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Nicotiana tabacum first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Nicotiana tabacum 2-acetolactate + CO2
-
?
pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Nicotiana tabacum ?
-
?