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Literature summary for 2.2.1.6 extracted from

  • Hill, C.H.; Pang, S.S.; Duggleby, R.G.
    Purification of Escherichia coli acetohydroxyacid synthase isoenzyme II and reconstitution of active enzyme from its individual pure subunits (1997), Biochem. J., 327, 891-898.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression is considerably higher from a vector that introduces a 50 residue N-terminal fusion containing an oligohistidine sequence on the large subunit Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
chlorimuron ethyl
-
Escherichia coli
chlorsulphuron
-
Escherichia coli
imazapyr
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
pyruvate native protein Escherichia coli
2.9
-
pyruvate fusion protein containing an N-terminal oligohistidine sequence on the large subunit Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16200
-
x * 16200 + x * 67200, SDS-PAGE Escherichia coli
67200
-
x * 16200 + x * 67200, SDS-PAGE Escherichia coli
119000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Escherichia coli first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
isoenzyme II
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme II Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
fusion protein with N-terminal oligohistidine at the large subunit Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Escherichia coli 2-acetolactate + CO2
-
?
pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 16200 + x * 67200, SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.4
-
pyruvate fusion protein containing an N-terminal oligohistidine sequence on the large subunit Escherichia coli
66.7
-
pyruvate native enzyme Escherichia coli