Cloned (Comment) | Organism |
---|---|
expression is considerably higher from a vector that introduces a 50 residue N-terminal fusion containing an oligohistidine sequence on the large subunit | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorimuron ethyl | - |
Escherichia coli | |
chlorsulphuron | - |
Escherichia coli | |
imazapyr | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
pyruvate | native protein | Escherichia coli | |
2.9 | - |
pyruvate | fusion protein containing an N-terminal oligohistidine sequence on the large subunit | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16200 | - |
x * 16200 + x * 67200, SDS-PAGE | Escherichia coli |
67200 | - |
x * 16200 + x * 67200, SDS-PAGE | Escherichia coli |
119000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Escherichia coli | first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoenzyme II | - |
Purification (Comment) | Organism |
---|---|
isoenzyme II | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53 | - |
fusion protein with N-terminal oligohistidine at the large subunit | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Escherichia coli | 2-acetolactate + CO2 | - |
? | |
pyruvate | first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16200 + x * 67200, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47.4 | - |
pyruvate | fusion protein containing an N-terminal oligohistidine sequence on the large subunit | Escherichia coli | |
66.7 | - |
pyruvate | native enzyme | Escherichia coli |