General Stability | Organism |
---|---|
dialysis causes 38% loss of activity of isoenzyme I, stimulates activity of isoenzyme III | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutanoate | isoenzyme I has lower sensitivity to inhibition than isoenzyme III | Escherichia coli | |
Val | isoenzyme I is more resistant to inhibition than isoenzyme III | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
pyruvate | isoenzyme I | Escherichia coli | |
7.6 | - |
pyruvate | isoenzyme III | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | Km: 0.3 mM for isoenzyme I, 0.4 mM for isoenzyme III | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Escherichia coli | - |
wild type and two isogenic strains PS1035, containing only acetohydroxy acid synthase III, and strain PS1036, containing only acetohydroxy acid synthase I | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Escherichia coli | 2-acetolactate + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
15 min, 69% loss of activity, isoenzyme I, slight stimulation of activity of isoenzyme III | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required by isoenzyme I, no requirement by isoenzyme III | Escherichia coli | |
FAD | Km: 0.00016 mM for isoenzyme I | Escherichia coli | |
thiamine diphosphate | required as cofactor | Escherichia coli | |
thiamine diphosphate | Km: 0.0087 mM for isoenzyme I, 0.026 mM for isoenzyme III | Escherichia coli |