Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 93% inhibition at 1 mM | Mycolicibacterium phlei | |
iodoacetic acid | 83% inhibition at 3.3 mM | Mycolicibacterium phlei | |
p-chloromercuriphenylsulfonic acid | complete inhibition at 1 mM | Mycolicibacterium phlei | |
Zn2+ | 97% inhibition at 1 mM | Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
2-oxoglutarate | - |
Mycolicibacterium phlei | |
3.2 | - |
glyoxylate | - |
Mycolicibacterium phlei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 31% activation at 1 mM | Mycolicibacterium phlei | |
Mn2+ | 175% activation at 1 mM | Mycolicibacterium phlei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium phlei |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2 | mechanism | Mycolicibacterium phlei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.21 | - |
- |
Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | - |
Mycolicibacterium phlei | ? + CO2 | - |
? | |
2-oxoglutarate + acetaldehyde | - |
Mycolicibacterium phlei | 5-hydroxy-4-oxohexanoate + CO2 | - |
? | |
2-oxoglutarate + glyoxylate | - |
Mycolicibacterium phlei | 2-hydroxy-3-oxohexandioate + CO2 | further spontaneous decarboxylation in the presence of acid | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay at | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Mycolicibacterium phlei |
6.5 | - |
enzyme assay at | Mycolicibacterium phlei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Mycolicibacterium phlei |