General Stability | Organism |
---|---|
glycerol, 20% w/v, stabilizes during purification | [Candida] boidinii |
glycerol, 20% w/v, stabilizes during purification | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii | - |
glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
D-xylulose 5-phosphate + formaldehyde | Ogataea angusta | - |
glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
D-xylulose 5-phosphate + formaldehyde | Ogataea angusta DL-1 | - |
glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
methanol-grown | - |
Ogataea angusta DL-1 | - |
methanol-grown | - |
[Candida] boidinii | - |
methanol grown | - |
Purification (Comment) | Organism |
---|---|
partial | [Candida] boidinii |
partial | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.285 | - |
- |
[Candida] boidinii |
Storage Stability | Organism |
---|---|
4°C, partially purified, inactivation within 12 h | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
D-xylulose 5-phosphate + formaldehyde | - |
Ogataea angusta | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
D-xylulose 5-phosphate + formaldehyde | - |
Ogataea angusta DL-1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
fructose 6-phosphate + formaldehyde | - |
[Candida] boidinii | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
fructose 6-phosphate + formaldehyde | - |
Ogataea angusta | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
fructose 6-phosphate + formaldehyde | - |
Ogataea angusta DL-1 | dihydroxyacetone + erythrose 4-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | markedly less activity in Tris than in phosphate or glycylglycine buffer | [Candida] boidinii |
7.4 | 7.6 | markedly less activity in Tris than in phosphate or glycylglycine buffer | Ogataea angusta |