Any feedback?
Literature summary for 2.2.1.3 extracted from
- Dihydroxyacetone synthase from Candida boidinii KD1 (1990), Methods Enzymol., 188, 435-445.
General Stability
| General Stability | Organism |
|---|---|
| dodecanethiol stabilizes most effectively | [Candida] boidinii |
| dodecanethiol stabilizes most effectively | Ogataea angusta |
| Mg2+ or EDTA stablizes | [Candida] boidinii |
| Mg2+ or EDTA stablizes | Ogataea angusta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 0.1 mM | [Candida] boidinii |  |
| Co2+ | 0.1 mM | Ogataea angusta | |
| formaldehyde | - |
[Candida] boidinii | |
| GSH | - |
[Candida] boidinii | |
| Mn2+ | 0.1 mM | [Candida] boidinii | |
| Mn2+ | 0.1 mM | Ogataea angusta | |
| NEM | - |
[Candida] boidinii | |
| Zn2+ | 0.1 mM | [Candida] boidinii | |
| Zn2+ | 0.1 mM | Ogataea angusta | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
[Candida] boidinii | 5777 | - |
| peroxisome | - |
Ogataea angusta | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | [Candida] boidinii | |
| additional information | - |
Ogataea angusta | |
| additional information | no activation by Ca2+ | [Candida] boidinii | |
| additional information | no metal cations | [Candida] boidinii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
alpha2, 2 * 76000, SDS-PAGE | [Candida] boidinii |
| 145000 | - |
gel filtration | [Candida] boidinii |
| 145000 | - |
PAGE | [Candida] boidinii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | Ogataea angusta | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii KD1 | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | Ogataea angusta DL-1 | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
methanol-grown | - |
| Ogataea angusta DL-1 | - |
methanol-grown | - |
| [Candida] boidinii | - |
- |
- |
| [Candida] boidinii | - |
methanol grown | - |
| [Candida] boidinii KD1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Candida] boidinii |
- |
Ogataea angusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
- |
[Candida] boidinii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, at least 2-3 weeks under 1 ml dodecanethiol | [Candida] boidinii |
| 4°C, at least 2-3 weeks under 1 ml dodecanethiol | Ogataea angusta |
| 4°C, inactivation within a few h in the absence of dodecanethiol | [Candida] boidinii |
| 4°C, inactivation within a few h in the absence of dodecanethiol | Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + D-glyceraldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + D-xylulose | - |
? | |
| D-xylulose 5-phosphate + D-glyceraldehyde | - |
[Candida] boidinii KD1 | glyceraldehyde 3-phosphate + D-xylulose | - |
? | |
| D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | - |
Ogataea angusta | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | Ogataea angusta | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii KD1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | [Candida] boidinii KD1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | - |
Ogataea angusta DL-1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | first step in formaldehyde assimilation via xylulose monophosphate (dihydroxyacetone) cycle | Ogataea angusta DL-1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + glycolaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one | - |
? | |
| D-xylulose 5-phosphate + glycolaldehyde | - |
[Candida] boidinii KD1 | glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one | - |
? | |
| D-xylulose 5-phosphate + ribose 5-phosphate | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + sedoheptulose 7-phosphate | - |
? | |
| D-xylulose 5-phosphate + ribose 5-phosphate | - |
[Candida] boidinii KD1 | glyceraldehyde 3-phosphate + sedoheptulose 7-phosphate | - |
? | |
| fructose 6-phosphate + formaldehyde | - |
[Candida] boidinii | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
| fructose 6-phosphate + formaldehyde | - |
Ogataea angusta | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
| fructose 6-phosphate + formaldehyde | - |
Ogataea angusta DL-1 | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
| hydroxypyruvate + formaldehyde | - |
[Candida] boidinii | CO2 + dihydroxyacetone | - |
? | |
| sedoheptulose 7-phosphate + formaldehyde | - |
[Candida] boidinii | dihydroxyacetone + ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 76000, SDS-PAGE | [Candida] boidinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 7.1 | [Candida] boidinii |
| 6.8 | 7.1 | - |
[Candida] boidinii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | tightly bound | [Candida] boidinii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
