General Stability | Organism |
---|---|
Mg2+, 5 mM, thiamine diphosphate 0.5 mM, DTT, 1 mM, EDTA 1 mM stabilize | [Candida] boidinii |
PMSF, 0.024% w/v, stabilizes | [Candida] boidinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
[Candida] boidinii | |
As2O3 | - |
[Candida] boidinii | |
Co2+ | - |
[Candida] boidinii | |
CuSO4 | - |
[Candida] boidinii | |
EDTA | - |
[Candida] boidinii | |
FeCl3 | - |
[Candida] boidinii | |
HgCl2 | - |
[Candida] boidinii | |
iodoacetate | - |
[Candida] boidinii | |
PCMB | - |
[Candida] boidinii | |
Zn2+ | - |
[Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
glyceraldehyde 3-phosphate | - |
[Candida] boidinii | |
0.43 | - |
formaldehyde | - |
[Candida] boidinii | |
0.52 | - |
dihydroxyacetone | - |
[Candida] boidinii | |
1 | - |
D-xylulose 5-phosphate | - |
[Candida] boidinii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | [Candida] boidinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
alpha4, 4 * 55000, SDS-PAGE | [Candida] boidinii |
190000 | - |
gel filtration | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii | pathway for formaldehyde fixation | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | - |
methanol grown | - |
[Candida] boidinii | - |
Kloeckera sp. No. 2201 | - |
Purification (Comment) | Organism |
---|---|
- |
[Candida] boidinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.88 | - |
- |
[Candida] boidinii |
Storage Stability | Organism |
---|---|
5°C, at least 1 month in buffer with stabilizing agents | [Candida] boidinii |
5°C, inactivation within 10 days with one of the stabilizing agents of the buffer omitted | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | r | |
D-xylulose 5-phosphate + formaldehyde | pathway for formaldehyde fixation | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | r | |
hydroxypyruvate + formaldehyde | efficiency as glycolaldehyde donor | [Candida] boidinii | CO2 + dihydroxyacetone | - |
? | |
additional information | with xylulose 5-phosphate as glycolyl donor: aliphatic aldehydes (C1-C7), glycolaldehyde and glyceraldehyde are better acceptors than ribose 5-phosphate | [Candida] boidinii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 55000, SDS-PAGE | [Candida] boidinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
[Candida] boidinii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | maximal activity at 35°C and about 80% of maximal activity at 45°C | [Candida] boidinii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min stable | [Candida] boidinii |
50 | - |
inactivation after 10 min | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
[Candida] boidinii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | about 80% of maximal activity at pH 6.5 and 7.5 | [Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
[Candida] boidinii |