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Literature summary for 2.2.1.3 extracted from

  • Kato, N.; Higuchi, T.; Sakazawa, C.; Nishizawa, T.; Tani, Y.; Yamada, H.
    Purification and properties of a transketolase responsible for formaldehyde fixation in a methanol-utilizing yeast, candida boidinii (Kloeckera sp.) No. 2201 (1982), Biochim. Biophys. Acta, 715, 143-150.
    View publication on PubMed

General Stability

General Stability Organism
Mg2+, 5 mM, thiamine diphosphate 0.5 mM, DTT, 1 mM, EDTA 1 mM stabilize [Candida] boidinii
PMSF, 0.024% w/v, stabilizes [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
[Candida] boidinii
As2O3
-
[Candida] boidinii
Co2+
-
[Candida] boidinii
CuSO4
-
[Candida] boidinii
EDTA
-
[Candida] boidinii
FeCl3
-
[Candida] boidinii
HgCl2
-
[Candida] boidinii
iodoacetate
-
[Candida] boidinii
PCMB
-
[Candida] boidinii
Zn2+
-
[Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
glyceraldehyde 3-phosphate
-
[Candida] boidinii
0.43
-
formaldehyde
-
[Candida] boidinii
0.52
-
dihydroxyacetone
-
[Candida] boidinii
1
-
D-xylulose 5-phosphate
-
[Candida] boidinii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
alpha4, 4 * 55000, SDS-PAGE [Candida] boidinii
190000
-
gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylulose 5-phosphate + formaldehyde [Candida] boidinii pathway for formaldehyde fixation glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone r

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
methanol grown
-
[Candida] boidinii
-
Kloeckera sp. No. 2201
-

Purification (Commentary)

Purification (Comment) Organism
-
[Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.88
-
-
[Candida] boidinii

Storage Stability

Storage Stability Organism
5°C, at least 1 month in buffer with stabilizing agents [Candida] boidinii
5°C, inactivation within 10 days with one of the stabilizing agents of the buffer omitted [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylulose 5-phosphate + formaldehyde
-
[Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone r
D-xylulose 5-phosphate + formaldehyde pathway for formaldehyde fixation [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone r
hydroxypyruvate + formaldehyde efficiency as glycolaldehyde donor [Candida] boidinii CO2 + dihydroxyacetone
-
?
additional information with xylulose 5-phosphate as glycolyl donor: aliphatic aldehydes (C1-C7), glycolaldehyde and glyceraldehyde are better acceptors than ribose 5-phosphate [Candida] boidinii ?
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 55000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
[Candida] boidinii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45 maximal activity at 35°C and about 80% of maximal activity at 45°C [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min stable [Candida] boidinii
50
-
inactivation after 10 min [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
[Candida] boidinii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 about 80% of maximal activity at pH 6.5 and 7.5 [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
[Candida] boidinii