Literature summary for 2.2.1.2 extracted from

Rale, M.; Schneider, S.; Sprenger, G.A.; Samland, A.K.; Fessner, W.D.
Broadening deoxysugar glycodiversity: natural and engineered transaldolases unlock a complementary substrate space (2011), Chemistry, 17, 2623-2632.

Search for more literature for 2.2.1.2

Protein Variants

Protein Variants	Comment	Organism
F178E	mutant is able to catalyze aldol reactions and readily accepts hihydroxypropanone and hydroxypropanone	Escherichia coli
F178E/S176A	improved acceptance of substrate hydroxypropanone compared with mutant F178E	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
D-Lactaldehyde	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.9	-	glyceraldehyde 3-phosphate	wild-type, pH 8.5, 30°C	Escherichia coli
2.4	-	glyceraldehyde 3-phosphate	mutant F178, pH 8.5, 30°C	Escherichia coli
59	-	D-glyceraldehyde	mutant F178, pH 8.5, 30°C	Escherichia coli
120	-	DL-glyceraldehyde	mutant F178, pH 8.5, 30°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydroxypropanone + D-glyceraldehyde	-	Escherichia coli	D-fructose	-	?
dihydroxypropanone + DL-glyceraldehyde	-	Escherichia coli	D-fructose	-	?
dihydroxypropanone + glyceraldehyde 3-phosphate	-	Escherichia coli	D-fructose 6-phosphate	-	?
additional information	enzyme catalyzes a stereospecifc carboligation resulting in D-threo configuration of the product. Donor substrates require a 1-hydroxy-2-alkanone moiety. No substrates: propanone, butanone	Escherichia coli	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.53	-	D-glyceraldehyde	mutant F178, pH 8.5, 30°C	Escherichia coli
0.54	-	DL-glyceraldehyde	mutant F178, pH 8.5, 30°C	Escherichia coli
12	-	glyceraldehyde 3-phosphate	mutant F178, pH 8.5, 30°C	Escherichia coli
13	-	glyceraldehyde 3-phosphate	wild-type, pH 8.5, 30°C	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4.3	-	DL-glyceraldehyde	mutant F178, pH 8.5, 30°C	Escherichia coli
9	-	D-glyceraldehyde	mutant F178, pH 8.5, 30°C	Escherichia coli
5000	-	glyceraldehyde 3-phosphate	mutant F178, pH 8.5, 30°C	Escherichia coli
6600	-	glyceraldehyde 3-phosphate	wild-type, pH 8.5, 30°C	Escherichia coli

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Release 2023.1 (January 2023)