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Literature summary for 2.2.1.2 extracted from

  • Soderberg, T.; Alver, R.C.
    Transaldolase of Methanocaldococcus jannaschii (2004), Archaea, 1, 255-262.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
D-ribose-5-phosphate above 15 mM Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0156
-
D-erythrose 4-phosphate pH 7.5, 25°C Methanocaldococcus jannaschii
0.0278
-
D-erythrose 4-phosphate pH 7.5, 50°C Methanocaldococcus jannaschii
0.64
-
D-fructose 6-phosphate pH 7.5, 25°C Methanocaldococcus jannaschii
0.65
-
D-fructose 6-phosphate pH 7.5, 50°C Methanocaldococcus jannaschii
2.2
-
D-ribose 5-phosphate pH 7.5, 50°C, concentration of ribose-5-phosphate between 0.5 and 15 mM Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23960
-
10 * 24000, SDS-PAGE, 10 * 23960, calculated Methanocaldococcus jannaschii
24000
-
10 * 24000, SDS-PAGE, 10 * 23960, calculated Methanocaldococcus jannaschii
271000
-
gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
pH 7.5, 25°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + D-erythrose 4-phosphate
-
Methanocaldococcus jannaschii ?
-
?
D-fructose 6-phosphate + D-ribose 5-phosphate
-
Methanocaldococcus jannaschii ?
-
?

Subunits

Subunits Comment Organism
decamer 10 * 24000, SDS-PAGE, 10 * 23960, calculated Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
3 weeks, no loss of activity Methanocaldococcus jannaschii
80
-
4 h, no loss of activity, 24 h, 50% residual activity Methanocaldococcus jannaschii
90
-
15 min, 20% loss of activity Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Methanocaldococcus jannaschii