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Literature summary for 2.2.1.10 extracted from
- L-Aspartate semialdehyde and a 6-deoxy-5-ketohexose 1-phosphate are the precursors to the aromatic amino acids in Methanocaldococcus jannaschii (2004), Biochemistry, 43, 7618-7627.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Mj0400, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | Methanocaldococcus jannaschii | 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | Q57843 | gene mj0400 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ | Methanocaldococcus jannaschii | ? | - |
? | |
| L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | the MJ0400 gene product catalyzes the transaldolase reaction | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | GC-MS analysis of reaction intermediates, overview. The enzyme is active as aldose with erythrose 4-phosphate and pyruvate producing a different stereoisomer from 3-deoxy-D-arabino-2-heptulosonate-7-phosphate | Methanocaldococcus jannaschii | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Methanocaldococcus jannaschii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Mj0400 is involved in the biosynthesis of aromatic compounds, including 3-dehydroquinate via an undetermined intermediate and 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, which is formed by NAD+-dependent Mj1249 from the intermediate compound. Also 4,5-dihydroxy-6-methylpipecolinic acid is formed from the intermediate. Pathway analysis, detailed overview | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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