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show all sequences of 2.2.1.10

MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity

Samland, A.K.; Wang, M.; Sprenger, G.A.; FEMS Microbiol. Lett. 281, 36-41 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene Mj0400, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS as mainly insoluble protein
Methanocaldococcus jannaschii
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-O-phosphonato-D-erythrose
competitive inhibition of aldolase activity
Methanocaldococcus jannaschii
glycylglycine
slightly inhibiting as buffer at 50 mM
Methanocaldococcus jannaschii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
MJ0400-His6 exhibits Michaelis-Menten kinetics in the aldolase reaction
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
Methanocaldococcus jannaschii
-
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
Q57843
gene mj0400
-
Purification (Commentary)
Commentary
Organism
soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity
Methanocaldococcus jannaschii
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
optimal growth temperature is 85°C
Methanocaldococcus jannaschii
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.033
-
purified recombinant enzyme, pH 7.5, 50°C, buffer Tris-HCl, HEPES/NaOH, or Na-phosphate
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
692386
Methanocaldococcus jannaschii
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
-
?
additional information
MJ0400 acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, catalyzing the reaction of L-aspartate semialdehyde and 6-deoxy-5-ketofructose-1-phosphate to 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, but the recombinant His6-tagged MJ0400 also catalyzes the cleavage of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, exhibiting fructose-1,6-bisphosphate aldolase, FBP aldolase, activity, EC 4.1.2.13, or the transaldolase reaction with D-fructose-6-phosphate and D-erythose-4-phosphate as substrates. The enzyme shows high substrate specificity for fructose-6-phosphate
692386
Methanocaldococcus jannaschii
?
-
-
-
-
pyruvate + D-erythrose 4-phosphate
-
692386
Methanocaldococcus jannaschii
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Methanocaldococcus jannaschii
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
85
10% activity at 25°C, higher activity at 50°C, optimal activity probably at or above the optimal growth temperature of 85°C
Methanocaldococcus jannaschii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 37 h
Methanocaldococcus jannaschii
100
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 1 h
Methanocaldococcus jannaschii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Methanocaldococcus jannaschii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.38
-
4-O-phosphonato-D-erythrose
recombinant enzyme, pH 7.5, 50°C
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
gene Mj0400, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS as mainly insoluble protein
Methanocaldococcus jannaschii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-O-phosphonato-D-erythrose
competitive inhibition of aldolase activity
Methanocaldococcus jannaschii
glycylglycine
slightly inhibiting as buffer at 50 mM
Methanocaldococcus jannaschii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.38
-
4-O-phosphonato-D-erythrose
recombinant enzyme, pH 7.5, 50°C
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
MJ0400-His6 exhibits Michaelis-Menten kinetics in the aldolase reaction
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
Methanocaldococcus jannaschii
-
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity
Methanocaldococcus jannaschii
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
optimal growth temperature is 85°C
Methanocaldococcus jannaschii
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.033
-
purified recombinant enzyme, pH 7.5, 50°C, buffer Tris-HCl, HEPES/NaOH, or Na-phosphate
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
692386
Methanocaldococcus jannaschii
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
-
?
additional information
MJ0400 acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, catalyzing the reaction of L-aspartate semialdehyde and 6-deoxy-5-ketofructose-1-phosphate to 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, but the recombinant His6-tagged MJ0400 also catalyzes the cleavage of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, exhibiting fructose-1,6-bisphosphate aldolase, FBP aldolase, activity, EC 4.1.2.13, or the transaldolase reaction with D-fructose-6-phosphate and D-erythose-4-phosphate as substrates. The enzyme shows high substrate specificity for fructose-6-phosphate
692386
Methanocaldococcus jannaschii
?
-
-
-
-
pyruvate + D-erythrose 4-phosphate
-
692386
Methanocaldococcus jannaschii
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Methanocaldococcus jannaschii
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
85
10% activity at 25°C, higher activity at 50°C, optimal activity probably at or above the optimal growth temperature of 85°C
Methanocaldococcus jannaschii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 37 h
Methanocaldococcus jannaschii
100
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 1 h
Methanocaldococcus jannaschii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Methanocaldococcus jannaschii
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in both carbon metabolism and amino acid biosynthesis, it catalyzes as transaldolase a step leading to biosynthesis of 3-dehydroquinate, which enters the shikimate pathway, and also shows activity as fructose 1,6-bisphosphate aldolase in carbon metabolism, overview
Methanocaldococcus jannaschii
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in both carbon metabolism and amino acid biosynthesis, it catalyzes as transaldolase a step leading to biosynthesis of 3-dehydroquinate, which enters the shikimate pathway, and also shows activity as fructose 1,6-bisphosphate aldolase in carbon metabolism, overview
Methanocaldococcus jannaschii
Other publictions for EC 2.2.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
692386
Samland
MJ0400 from Methanocaldococcus ...
Methanocaldococcus jannaschii
FEMS Microbiol. Lett.
281
36-41
2008
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718845
Morar
Structure of 2-amino-3,7-dideo ...
Methanocaldococcus jannaschii
Biochemistry
46
10562-10571
2007
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718838
White
L-Aspartate semialdehyde and a ...
Methanocaldococcus jannaschii
Biochemistry
43
7618-7627
2004
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