Literature summary for 2.2.1.1 extracted from

Sevostyanova, I.A.; Selivanov, V.A.; Yurshev, V.A.; Solovjeva, O.N.; Zabrodskaya, S.V.; Kochetov, G.A.
Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae (2009), Biochemistry (Moscow), 74, 789-792.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	D-ribose 5-phosphate	pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Ca2+	Saccharomyces cerevisiae
0.021	-	D-xylulose 5-phosphate	pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Ca2+	Saccharomyces cerevisiae
0.115	-	D-xylulose 5-phosphate	pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Mg2+	Saccharomyces cerevisiae
0.115	-	D-xylulose 5-phosphate	pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Mg2+	Saccharomyces cerevisiae
0.4	-	D-ribose 5-phosphate	pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Mg2+	Saccharomyces cerevisiae
0.4	-	D-ribose 5-phosphate	pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Mg2+	Saccharomyces cerevisiae
0.5	-	D-xylulose 5-phosphate	pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Ca2+	Saccharomyces cerevisiae
0.6	-	D-ribose 5-phosphate	pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Ca2+	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	in the presence of Ca2+, the enzyme exhibits strong negative active site cooperativity in both xylulose 5-phosphate binding and ribose 5-phosphate binding and positive cooperativity in the catalytic activity	Saccharomyces cerevisiae
Mg2+	nonequivalence of the active sites is not observed in presence of Mg2+	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate	in presence of Ca2+, the active centers of the enzyme are nonequivalent with respect to both ribose 5-phosphate and xylulose 5-phosphate binding	Saccharomyces cerevisiae	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?

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Release 2023.1 (January 2023)