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Literature summary for 2.2.1.1 extracted from

  • Domain, F.; Bina, X.R.; Levy, S.B.
    Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR (2007), Mol. Microbiol., 66, 383-394.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
hydrogen peroxide oxidative stress increases tktA expression. Induces tktA at 1 h treatment, while an increase in marRAB operon expression occurs only after 3 h exposure Escherichia coli

Application

Application Comment Organism
medicine TktA interacts with MarR. TktA decreases MarR repressor activity. Overexpressing tktA decreases antibiotic susceptibility. Endogenous TktA enhances multiple antibiotic resistance to a small degree through release of MarR repression of the marRAB operon Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET21b, tktA overexpressed using the high-copy-number expression plasmid pSPOK in Escherichia coli SPC105 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of tktA increases antibiotic and oxidative stress susceptibilities Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA resin-bound MarR Escherichia coli

Synonyms

Synonyms Comment Organism
TktA
-
Escherichia coli
transketolase A
-
Escherichia coli