Protein Variants | Comment | Organism |
---|---|---|
C160A | 81% of wild-type activity | Cereibacter sphaeroides |
C160D | 26% of wild-type activity | Cereibacter sphaeroides |
C160E | 7% of wild-type activity | Cereibacter sphaeroides |
C160S | 59% of wild-type activity | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
D-xylulose 5-phosphate | wild-type, pH 6.7, 25°C | Cereibacter sphaeroides | |
0.011 | - |
D-xylulose 5-phosphate | wild-type, pH 7.8, 25°C | Cereibacter sphaeroides | |
0.015 | - |
D-xylulose 5-phosphate | mutant C160A, pH 7.8, 25°C | Cereibacter sphaeroides | |
0.02 | - |
D-xylulose 5-phosphate | mutant C160D, pH 8.5, 25°C | Cereibacter sphaeroides | |
0.033 | - |
D-xylulose 5-phosphate | mutant C160S, pH 7.9, 25°C | Cereibacter sphaeroides | |
0.034 | - |
D-xylulose 5-phosphate | wild-type, pH 8.5, 25°C | Cereibacter sphaeroides | |
0.065 | - |
D-xylulose 5-phosphate | mutant C160E, pH 7.6, 25°C | Cereibacter sphaeroides | |
0.356 | - |
D-ribose 5-phosphate | wild-type, pH 6.7, 25°C | Cereibacter sphaeroides | |
0.436 | - |
D-ribose 5-phosphate | mutant C160S, pH 7.9, 25°C | Cereibacter sphaeroides | |
0.739 | - |
D-ribose 5-phosphate | mutant C160E, pH 7.6, 25°C | Cereibacter sphaeroides | |
0.837 | - |
D-ribose 5-phosphate | mutant C160D, pH 8.5, 25°C | Cereibacter sphaeroides | |
0.882 | - |
D-ribose 5-phosphate | mutant C160A, pH 7.8, 25°C | Cereibacter sphaeroides | |
0.949 | - |
D-ribose 5-phosphate | wild-type, pH 7.8, 25°C | Cereibacter sphaeroides | |
2.316 | - |
D-ribose 5-phosphate | wild-type, pH 8.5, 25°C | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
2 * 69000, SDS-PAGE | Cereibacter sphaeroides |
122000 | - |
gel filtration | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
overexpression in Escherichia coli | - |
Oxidation Stability | Organism |
---|---|
inactivation in presence of oxygen | Cereibacter sphaeroides |
Purification (Comment) | Organism |
---|---|
recombinant protein | Cereibacter sphaeroides |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate | equilibrium binding constants, first order rates of binding of cofactor | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.7 | - |
mutant C160A, pH 7.8, 25°C | Cereibacter sphaeroides |
9.5 | - |
mutant C160E, pH 7.6, 25°C | Cereibacter sphaeroides |
21.8 | - |
mutant C160S, pH 7.9, 25°C | Cereibacter sphaeroides |
30.1 | - |
mutant C160D, pH 8.5, 25°C | Cereibacter sphaeroides |
37 | - |
wild-type, pH 7.8, 25°C | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Cereibacter sphaeroides | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 69000, SDS-PAGE | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
mutant C160E | Cereibacter sphaeroides |
7.8 | - |
wild-type | Cereibacter sphaeroides |
7.8 | - |
mutant C160A | Cereibacter sphaeroides |
7.9 | - |
mutant C160S | Cereibacter sphaeroides |
8.5 | - |
mutant C160D | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | Km-value 0.0018 mM | Cereibacter sphaeroides |