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Literature summary for 2.2.1.1 extracted from

  • Bobst, C.E.; Tabita, F.R.
    The role of cysteine 160 in thiamine diphosphate binding of the Calvin-Benson-Bassham cycle transketolase of Rhodobacter sphaeroides (2004), Arch. Biochem. Biophys., 426, 43-54.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C160A 81% of wild-type activity Cereibacter sphaeroides
C160D 26% of wild-type activity Cereibacter sphaeroides
C160E 7% of wild-type activity Cereibacter sphaeroides
C160S 59% of wild-type activity Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
D-xylulose 5-phosphate wild-type, pH 6.7, 25°C Cereibacter sphaeroides
0.011
-
D-xylulose 5-phosphate wild-type, pH 7.8, 25°C Cereibacter sphaeroides
0.015
-
D-xylulose 5-phosphate mutant C160A, pH 7.8, 25°C Cereibacter sphaeroides
0.02
-
D-xylulose 5-phosphate mutant C160D, pH 8.5, 25°C Cereibacter sphaeroides
0.033
-
D-xylulose 5-phosphate mutant C160S, pH 7.9, 25°C Cereibacter sphaeroides
0.034
-
D-xylulose 5-phosphate wild-type, pH 8.5, 25°C Cereibacter sphaeroides
0.065
-
D-xylulose 5-phosphate mutant C160E, pH 7.6, 25°C Cereibacter sphaeroides
0.356
-
D-ribose 5-phosphate wild-type, pH 6.7, 25°C Cereibacter sphaeroides
0.436
-
D-ribose 5-phosphate mutant C160S, pH 7.9, 25°C Cereibacter sphaeroides
0.739
-
D-ribose 5-phosphate mutant C160E, pH 7.6, 25°C Cereibacter sphaeroides
0.837
-
D-ribose 5-phosphate mutant C160D, pH 8.5, 25°C Cereibacter sphaeroides
0.882
-
D-ribose 5-phosphate mutant C160A, pH 7.8, 25°C Cereibacter sphaeroides
0.949
-
D-ribose 5-phosphate wild-type, pH 7.8, 25°C Cereibacter sphaeroides
2.316
-
D-ribose 5-phosphate wild-type, pH 8.5, 25°C Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
2 * 69000, SDS-PAGE Cereibacter sphaeroides
122000
-
gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
overexpression in Escherichia coli
-

Oxidation Stability

Oxidation Stability Organism
inactivation in presence of oxygen Cereibacter sphaeroides

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Cereibacter sphaeroides

Reaction

Reaction Comment Organism Reaction ID
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate equilibrium binding constants, first order rates of binding of cofactor Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
mutant C160A, pH 7.8, 25°C Cereibacter sphaeroides
9.5
-
mutant C160E, pH 7.6, 25°C Cereibacter sphaeroides
21.8
-
mutant C160S, pH 7.9, 25°C Cereibacter sphaeroides
30.1
-
mutant C160D, pH 8.5, 25°C Cereibacter sphaeroides
37
-
wild-type, pH 7.8, 25°C Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Cereibacter sphaeroides sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 69000, SDS-PAGE Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
mutant C160E Cereibacter sphaeroides
7.8
-
wild-type Cereibacter sphaeroides
7.8
-
mutant C160A Cereibacter sphaeroides
7.9
-
mutant C160S Cereibacter sphaeroides
8.5
-
mutant C160D Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate Km-value 0.0018 mM Cereibacter sphaeroides