Crystallization (Comment) | Organism |
---|---|
cocrystallization of apotransketolase with 5 mM thiamine diphosphate, 5 mM CaCl2, 50 mM fructose-6-phosphate, 13-16% (w/W) polyethylenglycol 6000 in 50 mM glycyl-glycine buffer, pH 7.6, 0.0075 ml of a 20 mg/ml solution mixed with the same amount of mother liquid, space group: P212121 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D477A | lower activity than wild-type enzyme | Saccharomyces cerevisiae |
H469A | lower activity than wild-type enzyme | Saccharomyces cerevisiae |
R359A | lower activity than wild-type enzyme | Saccharomyces cerevisiae |
R528A | lower activity than wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
thiamine diphosphate | H469A | Saccharomyces cerevisiae | |
0.0006 | - |
thiamine diphosphate | wild-type | Saccharomyces cerevisiae | |
0.0012 | - |
thiamine diphosphate | D477A | Saccharomyces cerevisiae | |
0.0019 | - |
thiamine diphosphate | R359A | Saccharomyces cerevisiae | |
0.0024 | - |
thiamine diphosphate | R528A | Saccharomyces cerevisiae | |
0.073 | - |
D-xylulose 5-phosphate | wild-type | Saccharomyces cerevisiae | |
0.146 | - |
D-ribose 5-phosphate | wild-type | Saccharomyces cerevisiae | |
0.163 | - |
D-xylulose 5-phosphate | R359A | Saccharomyces cerevisiae | |
0.318 | - |
D-xylulose 5-phosphate | R528A | Saccharomyces cerevisiae | |
0.595 | - |
D-xylulose 5-phosphate | D477A | Saccharomyces cerevisiae | |
0.829 | - |
D-xylulose 5-phosphate | H469A | Saccharomyces cerevisiae | |
1.75 | - |
D-ribose 5-phosphate | D477A | Saccharomyces cerevisiae | |
5.65 | - |
D-ribose 5-phosphate | R359A | Saccharomyces cerevisiae | |
5.97 | - |
D-ribose 5-phosphate | H469A | Saccharomyces cerevisiae | |
7 | - |
D-ribose 5-phosphate | R528A | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74200 | - |
alpha2, 2 * 74200, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P23254 | baker's yeast | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 74200, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.8 | R359A, R528A | Saccharomyces cerevisiae |
6.6 | 7.8 | H469A | Saccharomyces cerevisiae |
6.8 | 7.8 | D477A | Saccharomyces cerevisiae |
7.6 | 8.2 | wild-type | Saccharomyces cerevisiae |