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Literature summary for 2.2.1.1 extracted from
- Immunoaffinity purification of rat liver transketolase: evidence for multiple forms of the enzyme (1986), Arch. Biochem. Biophys., 245, 212-219.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 10% v/v, stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Rabbit antibodies | not | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
D-xylulose 5-phosphate | (+ D-ribose 5-phosphate) | Rattus norvegicus |  |
| 0.72 | - |
D-ribose 5-phosphate | cosubstrate D-xylulose 5-phosphate | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
alpha2, 2 * 69000, SDS-PAGE | Rattus norvegicus |
| 139000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + D-ribose 5-phosphate | Rattus norvegicus | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immunoaffinity chromatography | Rattus norvegicus |
| partial, 5 multiple forms by isoelectric focusing | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, more than a week | Rattus norvegicus |
| -20°C, up to 2 months with 10% v/v glycerol | Rattus norvegicus |
| 0-4°C, several h | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 69000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
| 45 | - |
assay at, low enzyme concentration | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.2 | about half-maximal activity at pH 6.5 and 10.2 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
below, unstable | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Rattus norvegicus | |
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