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Literature summary for 2.2.1.1 extracted from
- Crystalline transketolase from bakers yeast: isolation and properties (1955), J. Biol. Chem., 214, 409-426.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + D-ribose 5-phosphate | Saccharomyces cerevisiae | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, crystalline, several weeks in 0.008 M glycylglycine buffer, pH 7.6 | Saccharomyces cerevisiae |
| room temperature, crystalline, in alkaline ammonium sulfate suspension | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 6-phosphate + D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | L-erythrulose | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | D-deoxyribose 5-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | glycolaldehyde | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | DL-glyceraldehyde | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | and acceptor substrates may be: D-glyceraldehyde 3-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | D-ribose 5-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| hydroxypyruvate + D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | CO2 + ribulose 5-phosphate | - |
? | |
| hydroxypyruvate + ribose 5-phosphate | - |
Saccharomyces cerevisiae | sedoheptulose 7-phosphate + ? | - |
? | |
| L-erythrulose + ribose 5-phosphate | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | no acceptors are D-ribose, formaldehyde, acetaldehyde, glucose 6-phosphate | Saccharomyces cerevisiae | ? | - |
? | |
| ribulose 5-phosphate + ribose 5-phosphate | ribulose is cleaved and ribose acts as acceptor | Saccharomyces cerevisiae | a heptulose phosphate + glyceraldehyde 3-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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