Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE3) RI cells | Sinomonas sp. No.22 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
(+)-sesamin | at pH 8.0 and 28°C | Sinomonas sp. No.22 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Sinomonas sp. No.22 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-demethylpiperitol + tetrahydrofolate | Sinomonas sp. No.22 | - |
(+)-didemethylpinoresinol + 5,10-methylenetetrahydrofolate | - |
? | |
(+)-sesamin + tetrahydrofolate | Sinomonas sp. No.22 | 100% activity | (+)-demethylpiperitol + 5,10-methylenetetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinomonas sp. No.22 | A0A1J1EM40 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Toyopearl butyl 650M column chromatography | Sinomonas sp. No.22 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.95 | - |
with piperine as substrate, at pH 8.0 and 28°C | Sinomonas sp. No.22 |
2.7 | - |
with (+)-episesamin as substrate, at pH 8.0 and 28°C | Sinomonas sp. No.22 |
7.7 | - |
with (-)-asarinin as substrate, at pH 8.0 and 28°C | Sinomonas sp. No.22 |
8.5 | - |
with (+)-sesamolin as substrate, at pH 8.0 and 28°C | Sinomonas sp. No.22 |
8.8 | - |
with (+)-sesamin as substrate, at pH 8.0 and 28°C | Sinomonas sp. No.22 |
9.8 | - |
with sesaminol as substrate, at pH 8.0 and 28°C | Sinomonas sp. No.22 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-demethylpiperitol + tetrahydrofolate | - |
Sinomonas sp. No.22 | (+)-didemethylpinoresinol + 5,10-methylenetetrahydrofolate | - |
? | |
(+)-episesamin + tetrahydrofolate | 31% activity compared to (+)-sesamin | Sinomonas sp. No.22 | 4-[(1S,3aR,4R,6aR)-4-(2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 5,10-methylenetetrahydrofolate | - |
? | |
(+)-sesamin + tetrahydrofolate | 100% activity | Sinomonas sp. No.22 | (+)-demethylpiperitol + 5,10-methylenetetrahydrofolate | - |
? | |
(+)-sesamolin + tetrahydrofolate | 97% activity compared to (+)-sesamin | Sinomonas sp. No.22 | 4-[(1S,3aR,4R,6aR)-4-[(2H-1,3-benzodioxol-5-yl)oxy]tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 4-[[(1R,3aR,4S,6aR)-4-(2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]oxy]benzene-1,2-diol + 4-[(1S,3aR,4R,6aR)-4-(3,4-dihydroxyphenoxy)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 5,10-methylenetetrahydrofolate | - |
? | |
(-)-asarinin + tetrahydrofolate | 88% activity compared to (+)-sesamin | Sinomonas sp. No.22 | 4-[(1S,3aS,4R,6aS)-4-(2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 4,4'-[(1R,3aS,4S,6aS)-tetrahydro-1H,3H-furo[3,4-c]furan-1,4-diyl]di(benzene-1,2-diol) + 5,10-methylenetetrahydrofolate | - |
? | |
piperine + tetrahydrofolate | 11% activity compared to (+)-sesamin | Sinomonas sp. No.22 | (2E,4E)-5-(3,4-dihydroxyphenyl)-1-(piperidin-1-yl)penta-2,4-dien-1-one + 5,10-methylenetetrahydrofolate | - |
? | |
sesaminol + tetrahydrofolate | 112% activity compared to (+)-sesamin | Sinomonas sp. No.22 | 4-[(1S,3aR,4S,6aR)-4-(6-hydroxy-2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 5-[(1S,3aR,4S,6aR)-4-(3,4-dihydroxyphenyl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2,4-triol + 5,10-methylenetetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 50000, SDS-PAGE | Sinomonas sp. No.22 |
homotrimer | 3 * 50385, calculated from amino acid sequence | Sinomonas sp. No.22 |
Synonyms | Comment | Organism |
---|---|---|
SesA | - |
Sinomonas sp. No.22 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Sinomonas sp. No.22 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | the enzyme is most stable at 10-30°C | Sinomonas sp. No.22 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
(+)-sesamin | at pH 8.0 and 28°C | Sinomonas sp. No.22 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Sinomonas sp. No.22 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | the enzyme is most stable in the pH range of 5.5-10.0 | Sinomonas sp. No.22 |
Organism | Comment | Expression |
---|---|---|
Sinomonas sp. No.22 | enzyme expression is inducible by sesamin | up |