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Literature summary for 2.1.5.1 extracted from

  • Kumano, T.; Fujiki, E.; Hashimoto, Y.; Kobayashi, M.
    Discovery of a sesamin-metabolizing microorganism and a new enzyme (2016), Proc. Natl. Acad. Sci. USA, 113, 9087-9092 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 CodonPlus (DE3) RI cells Sinomonas sp. No.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
(+)-sesamin at pH 8.0 and 28°C Sinomonas sp. No.22

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Sinomonas sp. No.22

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-demethylpiperitol + tetrahydrofolate Sinomonas sp. No.22
-
(+)-didemethylpinoresinol + 5,10-methylenetetrahydrofolate
-
?
(+)-sesamin + tetrahydrofolate Sinomonas sp. No.22 100% activity (+)-demethylpiperitol + 5,10-methylenetetrahydrofolate
-
?

Organism

Organism UniProt Comment Textmining
Sinomonas sp. No.22 A0A1J1EM40
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Toyopearl butyl 650M column chromatography Sinomonas sp. No.22

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.95
-
with piperine as substrate, at pH 8.0 and 28°C Sinomonas sp. No.22
2.7
-
with (+)-episesamin as substrate, at pH 8.0 and 28°C Sinomonas sp. No.22
7.7
-
with (-)-asarinin as substrate, at pH 8.0 and 28°C Sinomonas sp. No.22
8.5
-
with (+)-sesamolin as substrate, at pH 8.0 and 28°C Sinomonas sp. No.22
8.8
-
with (+)-sesamin as substrate, at pH 8.0 and 28°C Sinomonas sp. No.22
9.8
-
with sesaminol as substrate, at pH 8.0 and 28°C Sinomonas sp. No.22

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-demethylpiperitol + tetrahydrofolate
-
Sinomonas sp. No.22 (+)-didemethylpinoresinol + 5,10-methylenetetrahydrofolate
-
?
(+)-episesamin + tetrahydrofolate 31% activity compared to (+)-sesamin Sinomonas sp. No.22 4-[(1S,3aR,4R,6aR)-4-(2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 5,10-methylenetetrahydrofolate
-
?
(+)-sesamin + tetrahydrofolate 100% activity Sinomonas sp. No.22 (+)-demethylpiperitol + 5,10-methylenetetrahydrofolate
-
?
(+)-sesamolin + tetrahydrofolate 97% activity compared to (+)-sesamin Sinomonas sp. No.22 4-[(1S,3aR,4R,6aR)-4-[(2H-1,3-benzodioxol-5-yl)oxy]tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 4-[[(1R,3aR,4S,6aR)-4-(2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]oxy]benzene-1,2-diol + 4-[(1S,3aR,4R,6aR)-4-(3,4-dihydroxyphenoxy)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 5,10-methylenetetrahydrofolate
-
?
(-)-asarinin + tetrahydrofolate 88% activity compared to (+)-sesamin Sinomonas sp. No.22 4-[(1S,3aS,4R,6aS)-4-(2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 4,4'-[(1R,3aS,4S,6aS)-tetrahydro-1H,3H-furo[3,4-c]furan-1,4-diyl]di(benzene-1,2-diol) + 5,10-methylenetetrahydrofolate
-
?
piperine + tetrahydrofolate 11% activity compared to (+)-sesamin Sinomonas sp. No.22 (2E,4E)-5-(3,4-dihydroxyphenyl)-1-(piperidin-1-yl)penta-2,4-dien-1-one + 5,10-methylenetetrahydrofolate
-
?
sesaminol + tetrahydrofolate 112% activity compared to (+)-sesamin Sinomonas sp. No.22 4-[(1S,3aR,4S,6aR)-4-(6-hydroxy-2H-1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2-diol + 5-[(1S,3aR,4S,6aR)-4-(3,4-dihydroxyphenyl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]benzene-1,2,4-triol + 5,10-methylenetetrahydrofolate
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 50000, SDS-PAGE Sinomonas sp. No.22
homotrimer 3 * 50385, calculated from amino acid sequence Sinomonas sp. No.22

Synonyms

Synonyms Comment Organism
SesA
-
Sinomonas sp. No.22

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Sinomonas sp. No.22

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 30 the enzyme is most stable at 10-30°C Sinomonas sp. No.22

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.9
-
(+)-sesamin at pH 8.0 and 28°C Sinomonas sp. No.22

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Sinomonas sp. No.22

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10 the enzyme is most stable in the pH range of 5.5-10.0 Sinomonas sp. No.22

Expression

Organism Comment Expression
Sinomonas sp. No.22 enzyme expression is inducible by sesamin up